Chinese Journal of Blood Purification

12 September 2010, Volume 9 Issue 9 Previous Issue    Next Issue

述评

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Tracing the progress in erythropoiesis stimulating agents and iron metabolism for the guidance of anemia treatment in chronic ridney disease patients ZHENG Fa-lei;YUAN Qun-sheng 2010, 9 (9):  465-466.  Abstract ( 351 )   HTML ( 0 )   PDF (132KB) ( 204 )   Metrics

专题

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The present situation and prospect about the new type erythropoiesis stimulants XU Xiao-yi;CHEN Yi-pu 2010, 9 (9):  467-470.  Abstract ( 326 )   HTML ( 0 )   PDF (251KB) ( 166 )   Metrics

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Appropriate use of the rules for erythropoiesis stimulating agents in the treatment of anemia in chronic kidney disease SUN Xue-feng 2010, 9 (9):  470-472.  Abstract ( 332 )   HTML ( 0 )   PDF (202KB) ( 243 )   Metrics

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Hepcidin on the regulation of iron metabolism and its mechanism in chronic kidney disease YUAN Qun-sheng;LIU Peng-ju;ZHENG Fa-lei 2010, 9 (9):  473-476.  Abstract ( 349 )   HTML ( 0 )   PDF (276KB) ( 195 )   Metrics

临床研究

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The effects of erythropoietin on renal interstitial fibrosis and on the expression of MCP-1 and TGF-? in unilateral ureteral obstruction rats ZHAO Ban;WU Hua;ZHENG Fa-lei 2010, 9 (9):  477-481.  doi: 10.3969/j.issn.1671-4091.2010.09.00 Abstract ( 948 )   HTML ( 1 )   PDF (346KB) ( 326 )   【Abstract】Objective To examine the effect and to explore the mechanism of EPO on renal interstitial fibrosis in the rat model of unilateral ureteral obstruction. Methods Sixty male Wistar rats were divided into sham-operation group (n=20), unilateral ureteral obstruction (UUO) group (n=20), and EPO group (n=20) in which UUO rats were treated with EPO 100IU/kg three times a week. Rats were sacrificed at day 3, 7, 14 or 21 after the treatment. The extent of renal interstitial fibrosis was evaluated by Masson staining of the renal tissue. Immunohistological method and western blot were used to estimate the expression of transforming growth factor-β1 (TGF-β1), and monocyte chemoattractant protein-1 (MCP-1). Results The extent of interstitial fibrosis was significantly lower in EPO group at day 3 and 7 after the operation, compared with that in UUO group (p<0.05). The expression of MCP-1 and TGF-β1 in renal tissue was significantly lower in EPO group than that in UUO group at day 3, 7 and 14, respectively (p<0.05). Conclusions EPO may reduce the extent of renal fibrosis in the rat UUO model. This effect may relate to the down-regulation of TGF-β1 and MCP-1 expression, but the exact mechanism needs to be further studied. Metrics

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Iron metabolism state in maintenancehemo dialysis patients LI Han;WANG Shi-xiang 2010, 9 (9):  482-485.  doi: 10.3969/j.issn.1671-4091.2010.09.00 Abstract ( 829 )   HTML ( 0 )   PDF (251KB) ( 229 )   Objective To investigate the status of iron metabolism in maintenance hemodialysis (MHD) patients. Methods Seventy-six patients on MHD were involved in this cross-sectional study. The following data were collected: clinic data, serum ferritin and transferrin saturation, hemoglobin, and treatment of iron and erythropoietin preparations. t test and Chi-square test were used for statistical analyses. Results Among the 76 MHD patients, 36 (47.4%) MHD patients had iron deficiency. The prevalence of functional and absolute iron deficiency was 30.3% and 17.1%, respectively. The prevalence of serum ferritin <200ug/L, 200～800 ug/L, and >800ug/L was 22.4%, 52.6% and 25.0%, respectively. Four (21.1%) in 19 MHD patients with serum ferritin >800ug/L had functional iron deficiency. The level of hemoglobin in MHD patients with iron deficiency was (110.2±13.3)g/L, similar to the hemoglobin level of (111.3±10.9)g/L in MHD patients without iron deficiency. But the total EPO dose per week was significantly higher in MHD with iron deficiency than in MHD patients without iron deficiency [(7675.9±2108.9)U vs. (6429.1±1947.3)U, t=2.680, P=0.009]. The prevalence of iron deficiency was significantly lower and the prevalence of serum ferritin >800ug/L was significantly higher, in MHD patients receiving intravenous iron than in MHD patients receiving oral iron preparations. Conclusions The prevalence of iron deficiency in MHD patients was relatively high. The iron deficiency pattern was predominantly functional iron deficiency. The MHD patients with iron deficiency must be treated with higher dose of EPO to maintain their hemoglobin level. Intravenous iron administration was an effective method to reduce the prevalence of iron deficiency, but may result in the increase of serum ferrtin in MHD patients. Metrics

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A new parameter for the evaluation of volume status in hemodialysis patients: The peak value of impedance QI Hua-lin;MA Jun;WANG Wen-ting;ZHONG Ji-kang;XU Chen;YAN Hai-dong 2010, 9 (9):  486-489.  doi: 10.3969/j.issn.1671-4091.2010.09.003 Abstract ( 511 )   HTML ( 0 )   PDF (260KB) ( 188 )   【Abstract】 Objective In this study we summarized the changes of bioimpedance-frequency curve before and after hemodialysis with the multifrequency bioimpedance spectroscopy, and recommended a new parameter for the evaluation of volume status in hemodialysis patients Method Twenty healthy volunteers and 20 stable maintenance hemodialysis patients were included in this study. Whole-body bioimpedance was measured by using the spectroscopy with the frequency range between 100Hz and 50k Hz. The bioimpedance-frequency curves from hemodialysis patients were compared with those from healthy volunteers. Results The significance of bioimpedance-frequency curve was in accordance with that of the ECFC model. The impedance curve was significantly different between the patients before and after hemodialysis. The curve after hemodialysis was similar to that of healthy volunteers. The peak impedance value and peak frequency value were significantly different between pre- and post-hemodialysis, and between pre-hemodialysis and control group, but were indifferent between post-hemodialysis and control group. Peak impedance value was positively correlated with age, the ratio of weight to stature, and the change of weight before and after hemodialysis (r=0.631, p<0.01). Conclusion The peak impedance value is applicable for the evaluation of dry weight in hemodialysis patients. Metrics

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The effect of Benazepril and Losartan on serum potassium level in hemodialysis patients MA Li-jie;ZHOU Yi-Lun;SUN Fang;LIU Jing;SHEN Yang;HAN Bin;CUI Tai-gen 2010, 9 (9):  490-494.  doi: 10.3969/j.issn.1671-4091.2010.09.004 Abstract ( 680 )   HTML ( 0 )   PDF (315KB) ( 222 )   【Abstract】Objective To evaluate the effect of either combination therapy of Benazepril and Losartan or monotherapy of Benazepril or Losartan on serum potassium level in maintenance hemodialysis patients. Methods Forty-four hemodialysis patients with pre-dialytic serum potassium level lower than 6.0mmol/l were recruited. Patients were randomized into group A or group B. In group A, Benazepril (10mg q.d.) and Losartan (50mg q.d.) were sequentially added to the patients at the beginning and one month later. In group B, Losartan and Benazepril were sequentially added to the patients. Other medications remained unchanged. Serum potassium levels were measured at the beginning and 1, 2 and 3 months after start of the treatment. Fasting blood samples were collected in the morning at supine position, and serum aldosterone was measured by radioimmunoassay. Data were analyzed by t-test, analysis of variance test, or chi-square test to compare serum potassium level and the prevalence of hyperkalemia. Results There was no significant change in serum potassium level during the period of study in each group, nor was the prevalence of hyperkalemia; Serum aldosterone levels decreased significantly after the treatment (P=0.016). Conclusions Monotherapy of Benazepril or Losartan or combination therapy of Benazepril and Losartan are not associated with the higher prevalence of hyperkalemia in maintenance hemodialysis patients. Metrics

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A preliminary study on polyethersulfone floss membrane supporting the growth of L02 cell FAN Yi;WANG Ying-jie;CHEN Shu-lin;LI Gui-qing;ZHANG Shi-chang;LIU Tao;YU Xian-guo 2010, 9 (9):  495-497.  doi: 10.3969/j.issn.1671-4091.2010.09.005 Abstract ( 928 )   HTML ( 0 )   PDF (208KB) ( 190 )   【Abstract】Objective To observe whether polyethersulfone (PES) floss membrane is a suitable support membrane for hepatocyte L02 cultivation in vitro, and a possible scaffold to be used in the bioartificial liver support system. Methods L02 cells were inoculated with a density of 7.0 x 104 on PES floss membrane. By using suspended cell counting, MTT, confocal laser microscopy, scanning electron microscopy and RT-PCR methods, adherence ratio, cell viability, growth characteristics, AFP and CYP1A1 gene expression were evaluated and compared among the cells cultured on PES floss membrane, flat-membrane, and commonly used culture plate. Results ① L02 cells grew well on PES floss membrane. After inoculation for 10 hours, adherence ratio increased from 57.7% to 91.7% in L02 cells on PES floss membrane, which was higher than that of the cells cultured on flat membrane, but was similar to that of the cells on commonly used culture plate. Cell viability increased 4 or 5 times after 8 days, which was higher than that of the cells on other surfaces. ② Confocal microscopy showed that L02 cells adhered on PES floss membrane, grew as multi-cell aggregates, gradually connected by tight junctions, and formed 3 dimensional cell aggregates in some cells, which were confirmed by scanning electron microscopy. ③The expression of AFP and CYP1A1 genes in L02 cells growing on PES floss membrane was higher than that in the cells on flat membrane, but was similar to that in the cells on commonly used culture plate. Conclusion The PES floss membrane can effectively support adhesion, growth, proliferation, and expression of AFP and CYP1A1 of the L02 cells inoculated on its surface. Therefore, this membrane may be used as a novel scaffold biomaterial for bioartificial liver. Metrics

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Study on the inhibition effect of the short peptide AcSDKP on renal interstitial fibrosis in rats WANG Ming-ao;LIU Rui-chan;BAO Yu-shi;GU Yun-he;MU Su-hong;XIE Ru-juan 2010, 9 (9):  498-502.  doi: 10.3969/j.issn.1671-4091.2010.09.006 Abstract ( 870 )   HTML ( 0 )   PDF (401KB) ( 187 )   【Abstract】Objective To investigate the effect and mechanism of the short peptide acetyl-Ser-Asp-Lys-Pro (AcSDKP) on renal interstitial fibrosis induced by unilateral ureteral obstruction in rats. Methods Thirty-six male Wistar rats were randomly divided into sham-operation group, unilateral ureteral obstruction (UUO) group, and UUO+AcSDKP treatment group. Animal model of rat renal interstitial fibrosis was established by unilateral ureteral ligation. Rats were sacrificed after the surgery for 3 or 14 days. Histological change of the obstructed kidney was observed by H-E staining. The protein expression and localization of transforming growth factor β1 (TGF-β1) and -smooth muscle actin ( -SMA) in renal tissues were detected by immunohistochemistry. The mRNA levels of TGF-β1 and -SMA were measured by reverse transcription- polymerase chain reaction. Results Compared with sham-operated group, rats in UUO group exhibited severe renal interstitial fibrosis and higher expression of TGF-β1 and -SMA (P＜0.05), while those in UUO+AcSDKP group demonstrated less renal interstitial fibrosis, and lower expression levels of TGF-β1 and -SMA (P＜0.05). Conclusions AcSDKP treatment improves renal interstitial fibrosis in rats by down-regulating TGF-β1 and -SMA expression. Metrics

透析心理学

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The analysis of depression and its related factors in maintenance hemodialysis patients IANG Chun-sheng;LIU Ying;REN Ke;QIU Mo-yan;WANG Shao-hua;LUAN Jie 2010, 9 (9):  503-505.  doi: 10.3969/j.issn.1671-4091.2010.09.007 Abstract ( 529 )   HTML ( 0 )   PDF (189KB) ( 187 )   【Abstract】Objective We investigated the incidence of depression and its related factors in maintenance hemodialysis (MHD) patients. Methods A total of 122 MHD patients were divided into depression group or normal group based on Hamilton scale (HAMD). The authors analyzed depression related factors by comparing gender, age, payment type of medical expenses, marriage, education and employment between the two groups. Results Depression occurred in 66.4% MHD patients. There were no differences in gender, age, payment type of medical expenses, and marriage (P>0.05), but were significant differences in education level and employment status (P<0.05) between the two groups. Conclusion Depression is a common symptom in MHD patients. Depression is related to the education level and employment status of the MHD patients. Metrics