Abstract:

Objective To investigate the effect and possible mechanism of aldosterone receptor blocker and Angiotensin-converting Enzyme Inhibitor on peritoneal fibrosis. Methods 50 male Wistar rats were divided into three groups at random,Group A (n = 10) control group, B、C、D、E (n = 10) received daily intraperitoneal injection of 20 mL 4.25% Beter dialysate .on day 1、3、5、7, The rats was given intraperitoneal injection of LPS 0.6ml/(kg•h)Group B (n=10)modle groupC、D、E (n=10) medicine group, GroupC: angiotensin-converting enzyme inhibitor 10mg/(kg•d)was given orally to rats by gastric gavage in the morning once a day.GroupD:Aldosterone eceptor blocker. 100mg/(kg•d) was given orally to rats by gastric gavage in once a day.Thirty days later all rats were sacrificed. the parietal peritoneum was stained with HE and Masson. Total cell counts and macrophage counts in dialysate. To measure TGF-f abdominal cavity liquid of peritoneal dialysis rats. With ELISA method，and to measure the express of abdominal membrane MCP-1 and C-JUN with immunological histochemical method. Results TGF- obviously increased in Both B and C、D、E groups as compared to A group (P＜0.01) , group C、D is more serious than group E (P＜0.01), so do histopathology of peritoneum. Conclusions Aldosterone eceptor blocker and Angiotensin-converting Enzyme Inhibitor functions as an inhibitors of TGF-xpression in dialysate and suppressed MCP-1 expression in abdominal membrane possibly via down-regulation of c-Jun/activator protein-1. It demonstrate used Aldosterone eceptor blocker and Angiotensin-converting Enzyme at the same time more efficency than use one on inhibit Peritoneal fibrosis.

Key words: Aldosterone receptor blocker, Peritoneal fibrosis, Monocyte chemoattractant protein-1, C-Jun/activator protein-1