Abstract: Objectives To study the effect of PTFE hollow fiber tube attached with bovine serum albumin (BSA) on its outer layer on the adsorption of bilirubin. Methods For bilirubin adsorption test, we selected glytidy methacrylate (GMA) of 35% grafted rate and attached with BSA 75 mg per gram fiber MPTFE, centrifugal casting of the legal system into a plasma filter, having about 0.35m2 of filter area, and under the conditions of dark, 37℃ and 150-250ml/min plasma flow rate. Plasma circulated in the fiber tube. Due to the concentration gradient of bilirubin exterior and interior of PTFE tube, bound bilirubin in plasma dissociates from albumin, passes through the fenestrated membrane, and rebinds the BSA on the outer wall of the membrane, resulting in a new balanced bilirubin concentration across the membrane. We also observed the changes of plasma bilirubin concentration along with time. Results After the dialysis, plasma bilirubin concentration decreased gradually and significantly, reached the balanced concentration after 100 minutes with the bilirubin adsorption rate of about 15.26-32.11%. Conclusion The PTFE hollow fiber tube attached with bovine serum albumin (BSA) on its outer layer filters and adsorbs plasma bilirubin in the tube, resulting in the decrease of plasma bilirubin. The treated plasma is isolated from other plasma, and this method is therefore simpler than that using molecular absorption of recycling system (MARS). Dialysis using this tube is safer than the detoxication and adsorption procedure using plasma perfusion.

Key words: Polytetrafluoroethylene (PTFE) hollow fiber tube, Bovine serum albumin