Abstract: Objective To compare the serum petidome spectrum between uremic patients and normal controls, and to search for their variability. Methods The serum peptide profiling was determined in 10 uremic patients without dialysis, 10 uremic patients on peritoneal dialysis, 10 uremic patients on hemodialysis, and 12 healthy volunteers by the ClinProt magnetic bead enrichment and matrix assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS). Results Comparing to the data of normal controls, we screened out 7, 7 and 9 significantly expressed polypeptides in patients without dialysis, on peritoneal dialysis and on hemodialysis, respectively. t test was used for group comparisons, and the statistical significance was set at P＜0.05. Conclusions Our study established the serum petidome spectra for uremia by proteomic technology, and provided a new viewpoint to better understand the pathogenesis of uremia.

Key words: Serum peptide, ClinPro Tools, Proteomics