【Abstract】 Objective To comprehensively evaluate the safety, stability and validity of the portable continuous blood purification machine in continuous renal replacement treatment (CRRT) by animal experiments. Methods We conducted dog experiments to evaluate the safety, stability and validity of the portable continuous blood purification machine by running the machine for CRRT under a stable land environment and a simulated concussion environment for short period of time (8h), relatively long period of time (24h), and long period of time (72h). Results The safety and stability of the machine were perfect when it worked under a stable land environment and a simulated concussion environment for short and long period of time. The control of fluid replacement volume and dehydration accuracy fulfilled the national industrial standards. During the CRRT using this machine, the experimental dogs showed stable vital signs, decrease of serum K+, Cr and BUN (P＜0.05, as compared with those before the treatment), but no changes in serum Na+ and Cl- (P>0.05, as compared with those before the treatment). Conclusions The stability, safety and effectiveness of the portable continuous blood purification machine are satisfactory either under a stable land environment or under a simulated concussion environment.