Abstract: 【Abstract】Objective To test the different degree of glycosylation of serum IgA1 among MCNS-IgA, H-IgAN, MCNS, in order to investigate the relationship between MCNS-IgA and IgAN. Methods Sera from 30 patients were collected. 10 of them were MCNS-IgA, 10 patients were H-IgA and the rest were MCNS used as controls. Biotinylated lectins were used in ELISA to examine different glycans on serum IgA1 molecules. The α 2,6 sialic acid was detected by elderberry bark lectin (SNA), the exposure of terminal galactose (Gal) and N-acetylgalactosamine (GalNAc) were detected by arachis hypogaea (PNA) and Helix asperas agglutinin (HAA), respectively. The IgA1 glycans levels, corrected by IgA1 concentrations, were compared between patients and controls.  Results Reduced terminal α 2,6 sialic acid (1.232±0.250 vs. 0.756±0.243, P=0.015), and increased exposure of N-acetylgalactosamine (0.191±0.039 vs. 0.258±0.066, P=0.025) were demonstrated in serum IgA1 from patients with H-IgAN as compared in that from MCNS-IgA. However, MNCS and MCNS-IgA had no significant differences among the levels of glycosylation (P＞0.05).  Conclusion MCNS-IgA shows the characteristics of IgA nephropathy in serum IgA1 glycosylation level, suggesting the presence of MCD with non-specific mesangial IgA molecule deposition.

Key words: Minimal change nephrotic syndrome with mesangial IgA deposition, Serum IgA, Sialic acid, Galactose, N-acetylgalactosamine