Abstract: 【Abstract】Objective To observe the effects of Xuebijing on the inhibition of cell proliferation and the expression of high mobility group box-1 protein(HMGB-1) and transforming growth factor-β1(TGF-β1) in rat peritoneal mesothelial cells(PMCs) cultured in high glucose concentration. Methods PMCs were isolated from rat colic omentum and cultured in high glucose concentration(2.5%glucose). MTT method was processed to observe the effects of Xuebijing(2,10,20mg/mL)on the cell proliferation. ELISA analysis were performed to investigate the change of HMGB-1 and TGF--β1 concentration in the culture medium. Results High glucose concentration of 2.5% could significantly inhibit cell proliferation as well as induce the expression of HMGB-1 and TGF-β1 in PMCs（P<0.05），which could be partially reversed by Xuebijing of 2～20mg/mL（P<0.05）.Conclusion Xuebijing could reverse both the cell proliferation inhibition and cytokine like HMGB-1 and TGF-β1 induction by high glucose in PMC，and repair the cell damage induced by high glucose consequently.

Key words: Xuebijing, peritoneal mesothelial cell, proliferation, high mobility group box-1 protein, transforming growth factor-β1