Abstract: 【Abstract】Objective To investigate the protective effect of extrogenous hydrogen sulfide (H2S) on the injury of oxidative stress induced by high glucose in human peritoneal mesothelial cells line HMrSV5 cells. Methods HMrSV5 cells were incubated by 4.25% D-glucose (high glucose) to induce injury and cells were treated with a H2S donor sodium bisulfide (NaHS) for 24 h. MTT assay was used to detect the cell viability. The level of reactive oxygen species (ROS) was measured by flow cytometry. The level of lactate dehydrogenase (LDH) in the medium, The level of malondialdehyde (MDA) in the cells and the activity of superoxide dismutase (SOD) in the cells were measured. Results Compared with the control, the cell viability was singificantly decreased, the level of LDH in the medium was singificantly increased, the level of ROS in the cells was singificantly increased, the level of MDA in the cells was singificantly increased and the activity of SOD in the cells was singificantly decreased in high glucose group. Compared with the high glucose group, NaHS reversed the effect of high glucose. Conclusions Extrogenous H2S prevents the injury of oxidative stress induced by high glucose in human peritoneal mesothelial cells, which the mechanisms may be related to the inhibition of oxidative stress induced by H2S.

Key words: Human peritoneal mesothelial cells, High glucose, Oxidative stress, Lactate dehydrogenase, Reactive oxygen species, Malondialdehyde, Superoxide dismutase