Abstract: 【Abstract】 Objective To explore the strategy to increase the bacteria discovery rate in peritoneal dialysis fluid in a PD center. Methods A total of 466 peritonitis cases in the CAPD patients in the period of Jan. 2007 to Apr. 2014 were analyzed. Bacterial culture for dialysis fluid was performed using the traditional method for the CAPD patients before Jun. 2010 (original group), and using the method for blood culture for the CAPD patients in the period of Jun. 2010 to Apr. 2014 (improvement group). Antibiotic treatment, positive rate of bacteria, incidence of peritonitis, and causative bacteria were compared between the two groups. Results The ratio of antibiotic treatment before dialysis fluid culture was lower in improvement group than in original group (7.76% vs. 21.36%, P<0.01). The positive rate of bacteria in peritoneal dialysis fluid was 81.5％ in improvement group and was 65.8% in original group (P＜0.01). Conclusion The use of blood culture medium for the culture of dialysis fluid increased the positive rate of bacterial discovery in dialysis fluid. The use of antibiotics before bacterial culture for dialysis fluid profoundly affected the positive rate of bacteria in dialysis fluid.

Key words: Peritoneal dialysis related Peritonitis, Pathogen Culture method, positive rate