【Abstract】Objectives To evaluate the preliminary clinical outcome of early cannulation graft for arteriovenous fistula. Methods Thirty-seven cases with the early cannulation type graft (GORE® Acuseal) for arteriovenous fistula in the period from December 2016 to September 2018 were retrospectively analyzed. Their clinical data, the first cannulation time, patency and complications were summarized. Results There were 13 males and 24 females with an average age of 62.30+10.24 years. Thirty- one cases were in upper limbs and 6 cases in lower limbs. The success rate of the operation was 100%, and no perioperative complications happened. The first cannnulation time ranged from 14 hours to 42 days with an average cannulation time of 84.26+172.56 hours. Puncture-related subcutaneous hematoma was found in 3 cases. Mean follow-up period was 299.0±141.3 days with the follow-up rate of 100%. Thrombosis was found in 10 cases, stenosis in 6 cases, infection in 2 cases, and necrosis of local skin in 2 cases. Two cases died. No seroma, pseudoaneurysm or stealing syndrome occurred. The primary patency rates after 6 months and one year were 65.1%±8.2% and 46.9%±10.1% respectively; the primary assisted patency rates after 6 months and one year were 76.8%±7.2% and 69.6%±8.2% respectively; the secondary patency rate after one year was 94.6%±3.7%. Conclusions Early cannulation type of graft has the advantage of immediate cannulation after operation, avoiding central venous catheterization in transitional period or shortening the catheterization period. This type of graft is useful for clinical application.