Abstract: 【Abstract】Objective To observe the effect of extracorporeal immune cell intervention therapy(EICIT)on clinical and immunological indexes in a septic pig model. Methods The sepsis model was established by intraperitoneal injection of autologous incubated feces. After 24 hours, the pigs were treated with general fluid therapy (control group) or with EICIT in addition to the general fluid therapy (experimental group). Extracorporeal blood cell separation was conducted via right internal jugular vein catheter using the white blood cell acquisition program of the COBE Spectra Blood Cell Separator. Arterial blood gas, cytokine measurement, and immune cells by flow cytometry were performed at the points before building the model (T1), after building up the model for 24 hours but before treatment (T2), immediately after treatment (T3), and after treatment for 24 hours (T4). At the T4 time point, pathological examinations were performed under anesthesia and the animals were sacrificed. Results At T2 time point, we successfully established the sepsis model and the pigs showed lower mean arterial pressure, tachycardia, hypoxemia and higher blood lactic acid. Pro-inflammatory factors including procalcitonin (PCT), C-reactive protein (CRP), tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) increased, and interleukin (IL)-6 decreased significantly (t=-3.067, P=0.025), accompanied by a
reduced tendency of IL- 10. At T4 time point in the experimental group, hemodynamics parameters, partial pressure of blood oxygen, lactic acid level and infiltration of inflammatory cells improved significantly, as compared with those in the control group. In experimental group, the number of circulated CD8+ T-cells increased (t=4.373, P=0.006), granulocytic myeloperoxidase (MPO) activity became higher (t=3.637,P=0.005), but the numbers of CD4+T cells, B cells, dentric cells, monocytes and neutrophils had no significant changes. Conclusion We proved that EICIT combined with basic fluid treatment could improve the clinical symptoms of sepsis, including the improvement of hemodynamics, oxygenation status, blood lactic acid level, and the infiltration of inflammatory cells in organs in a septic pig model.

Key words: Sepsis, Acute kidney injury, Animal model, Extracorpereal immune cells intervention therapy（EICIT）