Abstract: Objective To investigate the effect of ischemic postconditioning on HIF-1Methods Seventy-two Sprague-Dawley rats were randomized into 3 groups: sham operation group (S group), ischemia-reperfusion group (IR group) and ischemic postconditioning group (IPO group). For rats in S group, we ligated the right renal artery, and separated and exposed the left kidney for 45min. For those in IR group, we ligated the right renal artery, and clamped the left renal artery for 45min. For those in IPO group, we also ligated the right renal artery and clamped the left renal artery for 45min, but we then sequentially loosed the clamp for 20sce and closed the clamp for 20sec for 10 cycles before recovery of the left kidney from ischemia. After the operation for 0.5h, 1h, 3h, 6h, 12h, 24h, 48h and 72h, rats were sacrificed, plasma creatinine and blood urea nitrogen were measured, and kidney samples were examined for HIF-1Results HIF-1ase at 12h. In IPO group, renal HIF-1Conclusion Ischemic postconditioning increases HIF-1

Key words: Ischemic postconditioning, HIF-1