Abstract: 【Abstract】Objective To explore the relationship between the 3 single nucleotide polymorphisms (SNPs) in three candidate genes, i.e., A163G in osteoprotegerin (OPG), Gln223Arg in leptin receptor (LEPR) and C161T in peroxisome proliferator-activated receptor (PPARγ), and the glucocorticoid-induced osteoporosis (GIO) in Chinese population. Methods We used the method of polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) to identify the SNPs of A163G in OPG, Gln223Arg in LEPR and C161T in PPARγ in 208 normal controls, 168 cases without GIO and 104 cases with GIO. Bone mineral density at lumbar spine and femoral neck was measured by using dual-energy X-ray absorption-metry (DEXA). Results By analysis of GIO with the respective SNPs, we obtained that the incidence of A163G in OPG and C161T in PPARγ as well as their related allele frequencies were significantly different between GIO cases and normal controls (p<0.05). When GIO was evaluated with the 3 SNPs, we found that the genotype rate of homologous GG at 163 in OPG in association with TT at 161 in PPARγ was significantly high in GIO cases (OR=2.04, 95%CI=1.26～3.67, P=0.02). Conclusion The SNPs of A163G in OPG and C161T in PPARγ may correlate with the glucocorticoid-induced osteoporosis in Chinese population, and these 2 SNPs may synergically interact in glucocorticoid-induced osteoporosis.

Key words: Single nucleotide polymorphism, Glucocorticoid, Bone mineral density