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Chinese Journal of Blood Purification

    12 October 2012, Volume 11 Issue 10 Previous Issue    Next Issue
    The prophylaxis strategy for contrast media induced acute renal injury
    LIANG Xian-hui, LIU Zhang-suo
    2012, 11 (10):  523-526.  doi: 10.3969/j.issn.1671-4091.2012.10.00
    Abstract ( 300 )   HTML ( 0 )   PDF (205KB) ( 360 )  
    AbstractObjective To explore the catheter-related infections in critical patients with blood purification.  Methods This is a retrospective analysis conducted by the Intensive Care Unit of Guangdong General Hospital. All patients admitted to the ICU during the 2 years period from January 1, 2010 to December 30, 2011 and treated with continuous renal replacement therapy (CRRT) or intermittent hemodialysis (IHD) were included. Results A total of 1,028 patients were included in this study. The total number of catheterization and catheterization days were 1,123 times and 16,845 days, including jugular catheterization 215 times and 3,665 days, femoral catheterization 908 times and 13,580 days, respectively. The total number of catheterization and catheterization days were 918 times and 14,688 days, respectively, in CRRT group, and were 295 times and 2,157 days, respectively, in IHD group. The incidence of catheter-related local infection (CRLI) was statistically higher in catheterization patients using femoral route than in those using jugular route (4.79 versus 2.72, P<0.05), but was indifferent between patients with CRRT and those with IHD (4.43 and 4.63, P>0.05). The incidence of catheter related blood stream infection (CRBSI) was statistically higher in catheterization patients using femoral route than in those using jugular route (3.24 versus 1.64, P<0.05), and was higher in patients with CRRT than those with IHD (3.13 and 1.85, P<0.05). Conclusion For critically ill patients required hemodialysis, blood access through internal jugular vein is preferable with the lower infection incidence than that through femoral vein. The incidence of CRBSI was higher in patients with CRRT than those with IHD. However, whether CRRT actually increases the CRBSI incidence needs to be further studied.
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    Comparison of the two insertion methods for Permcath catheter: a prospective cohort study
    LIU Ri-guang, MA Jin-hua, LI Jian-wen, CHEN Lin, CHEN Hao-xiong, CHEN Lei, FU Jun-zhou
    2012, 11 (10):  527-529.  doi: 10.3969/j.issn.1671-4091.2012.10.00
    Abstract ( 211 )   HTML ( 0 )   PDF (209KB) ( 213 )  
    AbstractObjectives To compare the safety and usability of the two insertion methods, pull-apart sheath method and guide wire method, for the insertion surgery of indwelling Permcath dialysis catheter.  Methods Two dialysis centers were involved in this study from September 2009 to December 2011. The patients who were arranged to use indwelling Permcath dialysis catheter were enrolled and divided into two groups, the use of pull-apart sheath method group (PAS group) and the use of guide wire group (WG group). Patients’ baseline information as well as the results of blood counts and blood coagulation tests were collected before and after the insertion surgery. Prospective observation was carried out since 24 hours after the insertion. The primary endpoint was the amount of blood loss during the insertion procedure; the secondary endpoints were the time spent for catheterization and the incidence of wound bleeding event within 24 hours after the insertion.  Results There were 22 patients in the PAS group and 23 patients in the GW group. The baseline information was basically similar between the two groups. Compared with the PAS group, the GW group had significantly less blood loss during insertion procedure (4.04±2.09ml vs. 12.86±3.35ml, P=0.000), shorter catheterization time (26.30±3.22min vs. 34.36±2.98min, P=0.000), and lower incidence of wound bleeding event within 24 hours after the insertion (4.358% vs. 27.27%, P=0.047). Conclusions The insertion of Permcath catheter by using guide wire can significantly reduced blood loss during insertion procedure, time spent for catheterization, and incidence of wound bleeding within 24 hours after the insertion, compared that by using pull-apart sheath method.
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    Bacterial distribution and risk factors for catheter-related bloodstream infection in hemodialysis patients
    AN Zi-min
    2012, 11 (10):  530-532.  doi: 10.3969/j.issn.1671-4091.2012.10.00
    Abstract ( 209 )   HTML ( 0 )   PDF (191KB) ( 269 )  
    AbstractObjective To explore the bacterial distribution and risk factors for catheter-related bloodstream infection in hemodialysis patients.  Methods We retrospectively studied 219 patients established a central venous catheter as vascular access for hemodialysis in our hospital from 2008 to 2011. The incidence of catheter-related bloodstream infection, the pathogen distribution and the related risk factors for the infection were analyzed. Results Thirty-four (15.52%) of the 219 patients with a central venous catheter suffered from catheter-related bloodstream infection, of which the main pathogens were Gram-positive bacteria (21 cases, 61.76%), including staphylococcus epidermidis 13 cases (38.23%) and staphylococcus aureus 5 cases (14.70%). The rate of catheter-related bloodstream infection was similar between the patients with femoral vein catheters and those with internal jugular vein catheters (χ2=0.914, P=0.339). Catheter-related bloodstream infection was positively related to indwelling time of catheters (χ2=13.350, P=0.001), but was unrelated to the underlying diseases (χ2=0.284, P=0.991). Conclusions The main pathogens responsible for the catheter related bloodstream infection were Gram-positive bacteria. The indwelling time of catheters was the risk factor for catheter-related bloodstream infection.
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    The utilization of intradermic transfixion to ligature attributive and communicating vein branches in the surgery of arteriovenous fistula
    WANG Yan-bin, ZHANG Hui, MENG Wei, HAN Guo-lin, XIANG Hua, CHANG Shuai
    2012, 11 (10):  533-536.  doi: 10.3969/j.issn.1671-4091.2012.10.00
    Abstract ( 147 )   HTML ( 0 )   PDF (269KB) ( 192 )  
    AbstractObjective To search for the best ligature technique of attributive and communicating vein branches in surgery of arteriovenous fistula (AVF).   Method The clinical data consisted of 98 patients who received AVF of radial artery and cephalic vein by end-to-end anastomosis. The course of radial artery and cephalic vein was detected by ultrasonography before surgery in order to identify the attributive and communicating branches of cephalic vein. The patients were randomly divided into two groups according to the number of vein branches needed to be ligatured; group I received intradermic transfixion (n=52) and group II received dissection and ligation (n=46) to ligature the vein branches. The items including operation time, complication of surgery, degree of tumescence, degree of pain, ratio of infection, efficiency of ligation, and ratio of induration were compared between the two groups. Results The operation time of group I was shorter than that of group II, and this difference enlarged as the number of ligatured vein branches increased (F=45.586, P=0.000). The degree of pain was less severe in group I than in group II (Z=2.289, P=0.022). The ratio and size of induration were less in group I than in group II (X2=4.176, P=0.041). The degree of tumescence showed no statistical difference between the two groups, but approximated to the statistically significant area (Z=1.904, P=0.057). There were no statistical differences between the two groups regarding surgery complications, infection ratio and ligation efficiency.  Conclusion The intradermic transfixion is better than dissection and ligation, and should be preferably used in the ligation of attributive and communicating vein branches in AVF surgery.
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    In-vitro stability and antimicrobial activity of vancomycin solution mixed with heparin
    CUI Rui, YANG Tao, LIU Ai-qing, ZENG Yi, LI Ming-ming, ZHANG Li-hong, LIU Li-fang, WANG Yu-zhu.
    2012, 11 (10):  537-540.  doi: 10.3969/j.issn.1671-4091.2012.10.00
    Abstract ( 215 )   HTML ( 0 )   PDF (209KB) ( 201 )  
    Abstract Objective We studied the in-vitro stability and antimicrobial activity of vancomycin solution mixed with heparin, to predict its efficacy when used in vancomycin/heparin lock manipulation to prevent catheter-related bacteremia among maintenance hemodialysis patients.  Methods Heparin was mixed with various concentrations of vancomycin, and stored at 37℃ for 0 min, 15 min, 30 min, 24 h, or 48 h. These mixtures were then examined for visual precipitation and clarity by a clarity instrument. One of the mixtures stored at 37℃ for 0 min, 15 min, 30 min, 24 h, and 48 h was assayed for the diameter of bacteriostatic circle against staphylococcus aureus (ATCC 29213), using the same vancomycin concentration in normal saline as the standard for comparison. Results Precipitates in the mixture reduced gradually with the decrease of vancomycin concentration, and disappeared completely in the mixtures containing <10 mg/ml vancomycin. The diameters of bacteriostatic circle from the mixtures containing 10 and 20 mg/ml vancomycin were similar to those from normal saline containing 10 and 20 mg/ml vancomycin, respectively (P>0.05). In addition, the diameters of bacteriostatic circle were statistically indifferent from one mixture stored at 37℃ for various period of time. Conclusions The degree of precipitation in vancomycin solution mixed with heparin is vancomycin concentration dependent. The mixture containing 10 mg/ml vancomycin is stable with sufficient bacteriostatic activity, and can be used as the vancomycin/heparin lock solution to prevent catheter-related bacteremia, especially that due to staphylococcus aureus infection.
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    The correlation analysis of serum calcium and phosphorus before and after total parathyroidectomy in maintenance hemodialysis patients
    XIAO Yue-fei, YANG Nai-ning, YAO Li, YANG Song-tao, WANG Yi-ping, GUO Xiu-zhen, WU Hong, LIU Zhan-xiao
    2012, 11 (10):  541-543.  doi: 10.3969/j.issn.1671-4091.2012.09.00
    Abstract ( 168 )   HTML ( 0 )   PDF (180KB) ( 242 )  
    AbstractObjective To explore the changes and related factors of serum calcium and phosphorus levels after total parathyroidectomy (TPTX) in maintenance hemodialysis (MHD) patients.  Methods A total of 30 MHD cases treated with TPTX were included in this study. Their total dosage of element calcium supplement within 10 days, serum intact parathyroid hormone (iPTH), serum alkaline phosphatase (ALP), serum calcium and phosphorus were measured before TPTX and after TPTX for 1, 4 and 7 days. Additionally, the volume of resected parathyroid was measured in 16 cases.  Results The pre-operative serum iPTH and ALP correlated positively with total dose of element calcium supplemented within 10 days after the operation (r=0.614, P<0.001 and r=0.385, P=0.036, respectively) and resected parathyroid volume (confirmed by pathological examination) (r=0.527, P=0.036 and r=0.799, P<0.001, respectively), and correlated negatively with post-operative serum calcium measured at every time point. After TPTX, serum calcium and phosphorus decreased gradually, and significantly decreased at 7th day (P<0.05). Conclusion After TPTX, serum calcium and phosphorus decreased gradually. The pre-operative serum iPTH and ALP correlated negatively to post-operative serum calcium, and positively to the total dose of element calcium supplemented after TPTX.
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    Clinical comparison of peritoneal dialysis catheter placement under laparoscope and by conventional surgical methods
    AO Xiang, ZHOU Qiao-ling, NIE Wan-nian, OU YANG Yan-lan.
    2012, 11 (10):  544-548.  doi: 10.3969/j.issn.1671-4091.2012.09.00
    Abstract ( 164 )   HTML ( 0 )   PDF (237KB) ( 225 )  
    AbstractObjective To compare the early and late complications, operation time, hospitalization day, and expenses for surgery and hospitalization between the placement of peritoneal dialysis (PD) catheters under laparoscope and by conventional operation method in patients with chronic kidney disease grade 5 (CKD grade 5). Methods A total of 357 patients diagnosed as CKD grade 5 were assigned into laparoscopic operation group (group A, n=141) and conventional operation group (group B, n=216) based on patients’ decision. In group A, the placement of PD catheter was carried out under laparoscope, and the distal end of the PD catheter was fixed by non-absorbable sutures. In group B, this manipulation was performed by using the conventional surgery of Seldinger technique. The differences in hemoglobin, platelet and serum creatinine before and after operation, operation time, early and later complications including bleeding, leakage, pain, peritonitis and catheter displacement, hospitalized day, and expenses for surgery and hospitalization were compared between the two groups.  Results Patients having past history of abdominal operation accounted for 27.7% in group A and 0.9% in group B (X2=59.977, P=0.000). Serum creatinine reduced after the operation in both group A (911.45±318.59 μmm/L vs. 875.42±316.34 μmm/L; t=-3.189, P=0.003) and group B (875.42±316.34 μmm/L vs. 816.25±270.12 μmm/L; t=2.007, P=0.049). The operation lasted 35.2±16.1 min in group A and 56.4±22.3 min in group B (t=6.224, P=0.014). The operation spent 5478.59±896.91 yuan in group A and 3446.26±1381.23 yuan in group B (t=7.675, p=0.000). Bleeding was found in 10.6% and 2.8% (t=9.521, P=0.002), pain in 0.7% and 11.1% (X2=14.174, P=0.000), catheter displacement within one month in 0% and 4.6% (X2=6.716, P=0.010), leakage within one month in 12.8% and 2.8% (X2=13.572, P=0.000), peritonitis within one month in 7.8% and 1.4% (X2=9.311, P=0.002) of the patients, respectively, in group A and group B. The incidences of leakage, catheter displacement within one year, peritonitis within one year and diarrhea, hospitalization day, hospitalization expense, and average hospitalization expense per day were indifferent between the two groups (P>0.05).  Conclusion PD improves renal function even performed after a short period of time. Placement of PD catheter under laparoscope is suitable for renal failure patients with abdominal operation history. It also has the advantages of shorter operation time, less pain and lower incidences of catheter displacement within one month than those from conventional surgical method. However, bleeding, leakage and peritonitis are frequently seen in placement of PD catheter under laparoscope.
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    A simple prediction model for the early patency rate of arteriovenous fistula 
    YAN Yan, XU Han, ZHAO An, TANG Jun, CHEN Qin-kai
    2012, 11 (10):  549-553.  doi: 10.3969/j.issn.1671-4091.2012.10.00
    Abstract ( 149 )   HTML ( 0 )   PDF (224KB) ( 255 )  
    Abstract Objective Method We recruited 1,051 end-stage renal disease patients in our hospital for this study. Their main factors and regression coefficients relating to AVF surgery were collected, to establish a risk score model based on the regression coefficient, to create a prediction score formula, and to evaluate their efficiencies. Results<0.0001). It appears that the prediction score of 103.17 may be an acceptable cut off value, yielding a sensitivity of 69.46%, specificity of 68.42%, positive predictive value of 98%, and negative predictive value of 9% in a subject population.  Conclusions The prediction score formula, established based on cephalic vein diameter, radial artery diameter, serum total cholesterol, the average diastolic blood pressure 2 days prior to the surgery to the morning of the surgery day, may be useful for the prediction of AVF early patency rate. Logistic regression analyses demonstrate that cephalic vein diameter (t=2.202, OR=2.085), radial artery diameter (t=3.493, OR=1.767), serum total cholesterol (t=-2.281, OR=0.708), and the average diastolic blood pressure 2 days prior to the surgery to the morning of the surgery day (t=2.723, OR=1.057) are the major factors relating to the success rate of AVF. The area under ROC curve based on individual total prediction scores calculated from risk score model is 0.707 (P To establish a simple prediction model for the early patency rate of arteriovenous fistula (AVF).
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    The role of zinc finger protein A20 on LPS-induced inflammation in rat peritoneal mesothelial cells
    ZOU Xun-liang, YAN Ju-zhen, XU Gang, WU Ping
    2012, 11 (10):  554-558. 
    Abstract ( 194 )   HTML ( 0 )   PDF (563KB) ( 162 )  
    AbstractObjective To investigate the role and the mechanism of zinc finger protein A20 (A20) on LPS-induced inflammation in cultured peritoneal mesothelial cells from Sprague-Dawley rats (RPMCs).  Method The second passage of cultured RPMCs were used in the study and randomly assigned into control group, LPS group, A20 plasmid transfection group, and parental plasmid transfection group. RPMCs were transfected with pGEM-T-easy-A20 plasmid using liposome for 12 hours, and were then stimulated with LPS. The cells and cell supernatant were harvested at different time after LPS stimulation. TLR4, p-IkBαand IkBα expressions in cells were determined by western blotting, and IL-18 in cell supernatant was determined by ELISA. Results In the A20 plasmid transfection group after LPS stimulation for 8h, the change of TLR4 expression was insignificant as compared with that in the control group (P=0.223), but was statistically different as compared with that in the LPS group and the parental plasmid transfection group (P=0.003 and 0.002, respectively). In the A20 plasmid transfection group after LPS stimulation for 1 h, p-IkBα expression changed insignificantly. The change of p-IkBα/IkBα ratio was insignificant as compared with that in the control group (p=0.553), but was statistically different as compared with that in the LPS group and parental plasmid transfection group (p=0.001 and 0.001, respectively). In A20 plasmid transfection group after LPS stimulation for 12 h, IL-18 secretion was higher (479.12±85.79 pg/ml) than that in the control group (274.34±47.21 pg/ml, P=0.012), but was lower than that in the LPS group (1049.45±185.01 pg/ml, P=0.011) and in the parental plasmid transfection group (1028.77±192.90 pg/ml, P=0.015).  Conclusions A20 inhibits the LPS induced inflammation in cultured RPMCs probably through negative regulation to several relevant proteins in the LPS signaling pathway.
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    Study on mortality of hemodialysis patients of Sichuan province in 2011
    HONG Da-qing, HE Qiang, PU Lei, DENG Fei, YANG Hong-ling, LI Gui-sen, WANG Li
    2012, 11 (10):  575-578. 
    Abstract ( 168 )   HTML ( 0 )   PDF (226KB) ( 273 )  
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