Abstract
【Abstract】 Objective To study the effects of glucose (0.1%、3.86%) on the production of IL-8 and MCP-1 in human peritoneal mesothelialcells（HPMC） in vitro and the role of endogenous reactive oxygen species (ROS) in this course. Methods The glucose (0.1%、3.86%) with or without hydrogen peroxide (0.1mmol/L、0.5mmol/L) 、3.86%mannitol、 glucose(3.86%) with pyruvate (35mmol/L) were used in culture medium to stimulate the HPMC. IL-8(Interlukin-8)mRNA and MCP-1(Monocyte chemoattractant protein-1)mRNA were measured by semiquantative RT-PCR, moreover, the protein level of IL-8 by ELISA. Results The glucose enhanced the production of IL-8 and MCP-1 following the increasing of concentration. On the contrary, the high osmolality mannitol had no these effects； Adding the antioxidant (pyruvate) in the glucose(3.86%), the expression of IL-8 was lowered, but not for the MCP-1. Conclusion The high glucose increases the expression of IL-8 and MCP-1, which may make a role in the uncomplicabality of dialysate and fibrosis of peritoneal membrane； The endogenous ROS can induce the expression of IL-8 as a signal message. Pyruvate can protect the HPMC by counterworking the endogenous oxidant stress.
Key words
Human peritoneal mesothelial cells(HPMC) /
Reactive oxygen species(ROS) /
Chemoattractant protein
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