Abstract:

【Abstract】 Objective To observe plasma level of TNFα in uremia and the influence of Cuprophane (Cup) and Hemophane (Hp) on production of tumor necrosis fact or alpha (TNFα) in maintenance hemodialysis (MHD) patients. Methods by in vitro incubation of peripheral blood monoe uclear cell (PBMC, LPS 10mg/L, 24h), ELISA and dot blot hybrodization to detect plasma TNFα levels, production and gene expression of TNFα by PBMC in 11 non-dialysis uremia patients, 14 MHD ones and 15 normal individuals. Results compared with normal control group, plasma TNFα level in uremic patients was higher, but was without significance (P>0.05). In MHD group equipped with Cup membrane, plasma TNFα level was elevated evidently with significance (P<0.05); after in vitro incubation of PBMC (LPS 10mg/L, 24h), the protein and gene expression of TNFα were higher than normal control either in non-dialysis patients or in MHD ones (P<0.05); During a hemodialysis(HD) session, the maximal amount of TNFα mRNA was detected at five minutes after the beginning of HD in group equipped with Cup (1.61±0.17 vs 0.84±0.06, and 1.61±0.17 rs 1.10±0.04 P<0.05), Simultaneously companion the increasing of protein levels of TNFα with significance. Conclusion Uremia and Cuprophane dialyer can stimulate PBMC to product TNFα.

Key words: Maintenance hemodialysis, Cuprophane tumor necrosis factor alpha