Objective To investigate the nutritional status and its influencing factors in hemodialysis (HD) patients. Methods Subjective global assessment (SGA) were used to assess the nutritional status in 57 HD patients. At the same time, the biochemical parameters, anthropometric datas and dietary assessments were also measured. The correlation between nutritional status and dietary intake, dialysis adequacy, dialysis duration and age were observed. Results There were 35 patients (61.4%) graded as of normal nutrition, 18 patients (31.6%) of mild malnutrition and 4 patients (7.0%) of severe malnutrition. Serum albumin (Alb), prealbumin (PA), creatinine (Scr), triceps skinfold thickness (TSF), dietary protein intake (DPI), and normalized protein catabolic rate (nPCR) were significantly different between the three groups, whereas Kt/V was not. The prevalence of malnutrition in HD patients aged 65 years or older was significantly higher than that in patients aged below 65 years old (50% vs. 32.4%). Conclusions There is a high morbidity of malnutrition in HD patients. Influencing factors include low protein intake, dialysis factors, age and inflammation.