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Chinese Journal of Blood Purification ›› 2003, Vol. 2 ›› Issue (5) : 240-243.

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Abstract

Objective To investigate the effect of different dialysis membranes on perepheral blood transforming growth factor-β1(TGF-β1) and the expression of complement regulatory protein CD59 in maintenance hemodialysis patients(MHD). Methods The levels of perepheral blood TGF-β1 and the expression of CD59 were measured by ELISA assay and flow cytometry in the patients with Cuprophone(CU) and Polysulfone(PSU). Results Compared with the lerels of TGF-β1［(49.3±6.1)ng/ml］in normal control group, the levels of TGF-β1 in MHD patients were significantly increased, but the levels of TGF-β1 in PSU group were significantly tower than that in CU group( P < 0.01). The perepheral blood mononuclear cell of normal person doesn't express CD59, but do in the group of uremia with or without hemodialysis. CD59 activity was lower in the no dialysis group, while higher in the dialysis group,and in CU group was significantly higher than that in PSU group( P<0.05). There is a positive correlation between TGF-β1 levels and CD59 expression both in CU group and PSU group. Conclusion Hemodialysis may activate complement by the reaction of blood and dialysis membrane,which induce the synthesize of TGF-β1 by perepheral blood mononuclear cells while abnormal high level of TGF-β1 would increase the activity of CD59, which inhibit cells damage caused by abnormal complement activation to protect bodies. But different dialysis membrane such as CU and PSU has different effect on TGF-β1 by and CD59 in MHD patients.