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Chinese Journal of Blood Purification ›› 2003, Vol. 2 ›› Issue (6) : 315-318.

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Abstract

Objective To investigate the effects of hepatopoietin (HPN) on the proliferation of rat glomerular mesangial cell(GMC) and on the production of transforming growth factorβ with the stimulation of interleukin-1β in vitro.
Methods GMC proliferation was determined by 3H-TdR incorporation. TGFβ level in the culture supernatant was measured by ELISA. Results HPN on the dosage of 400u/well only inhibited the rat GMC proliferation （P<0.01） and had no influence on TGFβ production without the existence of IL-1β, but it influenced both with the existence of IL-1β（respectively, P<0.01）. It didn't show above actions on the dosage of 100 u/well whether with the existence of IL-1β or not. Conclusion HPN could significantly inhibit rat GMC prolifera-tion and the production of TGFβ induced by IL-1β in high dosage in vitro. The action of HPN has no species and organic specificity.