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Chinese Journal of Blood Purification ›› 2004, Vol. 3 ›› Issue (10) : 546-548.

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Abstract

Objective To study the expression of BcL-2 and IL-2 on peripheral blood lymphocytes in the patients with Hemodialysis(HD), and the relationship between these changes with the impaired immunity in longterm hemodialysis patients. Methods Twenty-seven hemodialysis patients and 25 normal controls were investigated. The expression of CD3-FITC/BcL-2-PE and IL-2R on peripheral blood lymphocytes (PBL) were detected by flow cytometry.The contents of sIL-2R were measured by ELISA method. Pripheral blood samples were collected before and right after hemodialysis. Results ①The activation-induced cell death occured in the hemodialysis patients; ②The Bcl-2 expression of HD patients before HD was(11.2±0.79)% witch is significantly lower than that of normal controls(38.94 ±0.76)%, P<0.01, the BcL-2 level of the patients increased lightly after hemodialysis; ③The changes of cell factors: The IL-2R and sIL-2R level were (9.78±1.21)% and (813.15±134.78)% respectively, both were significantly higher than that of normal controls (P<0.01), After hemodialysis,the level of IL-2R decreased,while sIL-2R was still 3 times higher than the normal range.The content of IL-2 was (54.21±31.5)ku/L before and (68.76±27.55)ku/L after hemodialysis (P<0.05), the changes of sIL-2R were significantly positively related to the Scr level (P<0.05), and the changes of Bcl-2 was significantly positively related to IL-2 level. Conclusion The aberration of Bcl-2 and IL-2 expression may accelerate apoptosis of HD patients' PBL. It is involved in the pathogenesis of immuno-suppression.