Objective To study the immune modulation mechanism of renal tubule assist device (RAD) in end-stage renal disease (ESRD) therapy. Methods Cultured with serum of sepsis or medium contain lipopolysaccharide(LPS) on human kidney tuble epithelial cell lines HKC，the transcription and translation expression of interleukin-10(IL-10) were detected with RT-PCR、ELISA. The translocation of IL-10 in HKC stimulated with LPS was detected with "transwell". Results The expression of IL-10 showed higher in HKC from renal tubule assist device stimulated with LPS than that in normal culture medium or ultrafiltrate. The HKC can secret IL-10 toward both apical and basal. Conclusion The HKC cells in RAD take part in immune regulation with the expression of IL-10 stimulated by LPS.