Objective To evaluate the effect of staphylococcal protein A(SPA) adsorption therapy on panel reactive antibody（PRA）. Methods The experiment of SPA adsorption-system was carried out in vitro and in vivo for 32 sensitive recipients ［Population reactive antibody (PRA)>25%］ patients and clinical kidney transplantation of these patients were observed. Results ①About 450～600ml blood plasma was given out in one SPA adsorption treatment. The mean levels of IgG adsorbtion saturated was (3.98 ± 0.51)g/L; at the end of the experiment the mean level was (19.54 ± 3.13)g/L. The levels of plasma IgG after absorption was decreased by 51.2%; ②Before SPA adsorption therapy, the levels of PRA were: 98%～51% for 5cases、50%～40% for 15 cases and 39%～25% for 12 case. After (6.70± 3.11) times SPA adsorption therapy, the PRA levels decreased to lower than 12% in 29 patients（90.62%）and 7 000～9 000ml circulated plasma involved in every SPA adsorption therapy. ③Twenty-seven cases（83.37%）accepted kidney transplantation, no serious rejection occurred and all recovered. Conclusions To sensitive patients, the levels of PRA could be reduced by the SPA adsorption therapy which had a definitely therapeutic effects on the acute rejections following renal transplantation if combined with better HLA matching model of kidney and new type immunosuppressants.