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Chinese Journal of Blood Purification ›› 2005, Vol. 4 ›› Issue (11) : 601-604.

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Abstract

Objective To explore the important role of erythropoietin receptor (EPOR) in the mechanisms of renal anemia via observing the change of EPOR of rat bone marrow and analyzing the relations between graft function and anemia after renal transplantation. Methods The chronic renal failure(CRF) rats were surgically induced by 5/6 nephrectomy. CRF rats were recipients of renal allograft. The expression of EPOR mRNA and the content of EPOR protein of bone marrow were examined by RT-PCR technique and Western Blot analysis pre- and post-transplant. Correlations between EPOR mRNA, the content of EPOR protein, serum creatinine(Scr), and hemoglobin(Hb) were analysed. Results CRF model were successfully established on post operation days (POD) 90. The Scr and BUN decreased sharply immediately after allograft transplantation. Expression of EPOR mRNA and content of EPOR protein of CRF rats were significantly lower than normal level. After transplantation, expression of EPOR mRNA and content of EPOR protein increased significantly on POD 7 and recovered on POD 14. Negative correlations were found between the expression of EPOR mRNA or content of EPOR protein and Scr respectively, whereas statistically positive correlations were found between the expression of EPOR mRNA or content of EPOR protein and Hb. Conclusions EPOR mRNA and the content of EPOR protein recovers to normal level after allograft transplantation when uremic toxins are eliminated completely. It indicated that the decrease of EPOR of CRF rats is an important mechanism of renal anemia.