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Chinese Journal of Blood Purification ›› 2007, Vol. 6 ›› Issue (1) : 24-28.

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Abstract

Objective To investigate the effect of polymorphism(A163G) of promoter region of osteoprotegerin(OPG) gene on bone mineral density(BMD) and markers of bone turnover in patients using glucocorticoid. Methods The A163G genotypes of OPG promoter region were determined by PCR-RFLP in 208 healthy persons and 272 patients using a large dose of glucocorticoid,and BMD at lumbar spine,femoral neck,trochanter and Ward’ triangle etc, were measured by dual-energy X-ray absorptiometry(DEXA). Some markers of bone turnover were also detected. Results Hardy-Weinberg equilibrium was evident for OPG polymorphism. The frequency of the genotype had no significant difference between the two groups. According to bone mineral density of lumbar spine, patients using glucocorticoid were divided into osteopenia group and osteoporosis group, The frequency of the genotype was significantly different between the two groups.In groupⅡ, the BMD at lumbar spine area was (1.04±0.12)g/cm2, (0.98±0.09)g/cm2， (0.83±0.08)g/cm2 of AA,AG,GG genotypes respectively, the BMD at femoral neck area was(0.84±0.09)g/cm2,(0.75±0.07)g/cm2，(0.69±0.09)g/cm2 of AA,AG,GG genotypes respectively, among which a significant difference was observed(P＜0.05 by ANOVA), and the significant difference also presented after adjusted by age and BMI, but not found the significant difference in other markers. In group I, significant link was not observed between OPG polymorphism and BMD with markers of bone turnover. Conclusion The frequency of the genotype was no significant difference between the two groups，it has no relationship with onset of glomerulonephritis. The frequency of the genotype is significantly different between osteopenia group and osteoporosis group, it has relationship with onset of glucocorticoid induced-osteoporosis. polymorphism(A163G) of OPG gene may have an effect on the BMD of femoral neck and lumbar spine of the patients using hormone. Allele A may be the protection factor of bone mass, it has possible relationship with the loss of bone mass.