Abstract: Objective To observe the effect of BL-300 perfusion column on clearance of biochemical substances in plasma for patients with chronic severe hepatitis. Methods Mixed plasma collected from patients with chronic severe hepatitis flowed through BL-300 perfusion column in cycles. Serum biochemical substances including total bilirubin (TBIL), direct bilirubin (DBIL), bile acid (TBA), albumin (ALB) and globulin (GLB) were tested before the perfusion and after the pass-through reached 32.0 liters. The clearance rate of TBIL and DBIL was calculated when the total amount of pass-through was 0, 0.5, 1.0, 2.0, 4.0, 8.0, 16.0 and 32.0 liters, respectively. We also observed the changes of these serum biochemical parameters in 65 patients with chronic severe hepatitis treated with hemoperfusion using the BL-300 perfusion column. Results When the total amount of pass-through reached 32.0 liters, TBIL, DBIL and TBA in the mixed plasma decreased significantly (P＜0.01, as compared with those in the plasma prior to perfusion), and ALB and GLB decreased slightly (P＜0.05). TBIL concentration decreased by 58.1%, DBIL by 54.9%, TBA by 86.9%, ALB by 7.5% and GLB by 11.7%, corresponding to the total adsorbed amount of 342.2μmol, 292.3μmol, 263.5μmol, 7.0g and 9.5g, respectively. The clearance rate of TBIL and DBIL of the mixed plasma decreased gradually with the increase of total amount of pass-through. In patients with chronic severe hepatitis after perfusion with BL-300 perfusion column, serum biochemical parameters showed the same changes as those of the mixed plasma. No other abnormal changes were found in this study. Conclusion BL-300 perfusion column is effective in adsorption of serum TBIL, DBIL and TBA in patients with chronic severe hepatitis. Supplement of ALB and GLB is necessary to the patients treated with artificial liver of this column.

Key words: Severe hepatitis, Artificial liver