A preliminary study of polyethersulfone membrane with a single-layer skin supporting the growth of HepG2 cells

YANG Guang-hui; ZHANG Shi-chang; WANG Ying-jie; LIU Tao; SUN Shu-dong

PDF(514 KB)

Chinese Journal of Blood Purification ›› 2008, Vol. 7 ›› Issue (12) : 651-654.

基础研究

A preliminary study of polyethersulfone membrane with a single-layer skin supporting the growth of HepG2 cells

YANG Guang-hui1, ZHANG Shi-chang1, WANG Ying-jie1, LIU Tao1, SUN Shu-dong

Author information +

History +

Abstract

Objective To evaluate whether polyethersulfone (PES) membrane is capable of supporting hepatocyte growth and suitable to be used as the bioartificial liver support systems. Methods The structure of PES plate membrane was observed microscopically. HepG2 cells grew on the PES membrane, and their morphology, growth curve, albumin secretion and the transformation by pentoxyresorufin were examined. Results Prepared PES plate membrane demonstrated a single-layer skin, porous and asymmetric structure. HepG2 cells cultured on PES membrane showed a typical growth curve and proliferated to 6-7 folds after 8 days. These cells clustered together and covered the surface of the membrane, even extended into the micropores. LDH leakage had no difference between the HepG2 cells growing on the membrane and those of the control group. HepG2 cells growing on the membrane showed higher protein biosynthesis and biotransformation functions. Conclusion The PES membrane is a substrate suitable for hepatocytes growing on it. It may be useful for the construction of hollow fiber bioreactors.

Key words

Single-layer skin / HepG2 cells / Hollow fiber

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

YANG Guang-hui;ZHANG Shi-chang;WANG Ying-jie;LIU Tao;SUN Shu-dong. A preliminary study of polyethersulfone membrane with a single-layer skin supporting the growth of HepG2 cells[J]. Chinese Journal of Blood Purification. 2008, 7(12): 651-654