Abstract: 【Abstract】Objectives To investigate the expression of pHK-shRNA plasmid mediated by polyamidoamine dendrimers（PAMAM）in mouse peritoneum. Methods Kunming mice were randomly divided into transfection group and control group, and were intraperitoneally injected with PAMAM G9/pHK-shRNA complex and saline, respectively. Parietal and visceral peritoneum were collected after the injection for 24h, 48h, 96h and 7d, and the expression of green fluorescent protein (GFP) in peritoneum was detected by fluorescence microscope and western blot. Results Expression of GFP was not detected in control group, and was detected in peritoneum of transfection group at all time points. Western blot and observation of tissue sections showed that the peak of the expression was at 48h after the transfection. Conclusions pHK-shRNA plasmid can be efficiently transferred into mouse peritoneal tissue by PAMAM, which provides an approach for in vivo experimental studies.

Key words: Peritoneal tissue, Transfection