Objective: To investigate the effects of dialysis with balancing amino acid dialysate in chronic hemodialysis patients. Methods: Twenty-nine chronic hemodialysis patients were dialysis treated with balancing amino acid dialysate for one month. Serum free amino acid, plasma proteins, some markers of inflammation, BUN, Cr, electrolytes were measured before and after treatment. Results: After dialysis with balancing amino acid dialysate for one month, the levels of most kinds free amino acids and the level of total amino acid elevated to normal, and the levels of BUN, SCr and UA elevated. Neither the concentrations of electrolytes, calcium and P , nor the levels of plasma total protein, ALB PreA asn Trf had any significant changes. The serum levels of β2-MG和SCRP decreased significantly, compared with baseline levels (P<0.05). Conclusion: The dialysis with balancing amino acids might prevent the loss of amino acids, improve the metabolic disorder, and inflammatory status in the chronic hemodialysis patients.