ffect of glucose degradation products on AQP1 and eNOS expression in cultured endothelial cells

LIN Xing-hui; QIAN Jia-qi; NI Zhao-hui; Alessandro Amore

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Chinese Journal of Blood Purification ›› 2008, Vol. 7 ›› Issue (8) : 424-426.

基础研究

ffect of glucose degradation products on AQP1 and eNOS expression in cultured endothelial cells

LIN Xing-hui1, QIAN Jia-qi1, NI Zhao-hui1, Alessandro Amore2, Rosanna Coppo

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Abstract

Objective To analysis the effect of glucose degradation products (GDPs) on aquaporin-1(AQP1) and endothelial nitric oxide synthase (eNOS) expression in cultured endothelial cells. Methods Endothelial cell line (t End.1) was incubated for 24 hours with two relevant GDPs - methylglyoxal (MGly) or 2-furaldehyde (Fur) at the concentrations reported in traditional peritoneal dialysis fluid (Fur 0.8 μM; MGly 35 μM). AQP1 and eNOS gene expression was detected by reverse transcription-polymerase chain reaction (RT-PCR). AQP1 protein was detected by western blot. Moreover, the effect of GDPs on AQP1 gene expression was assayed in time- and dose-dependent manners. Results Fur and MGly at the concentrations reported in traditional peritoneal dialysis fluid (Fur 0.8μM; MGly 35μM) significantly up-regulated eNOS mRNA and tended to down-regulate AQP1 mRNA in cultured endothelial cells. Western blot revealed similar results. Conclusion GDPs stimulate eNOS expression in endothelial cells without up-regulating AQP1 expression. These effects may be involved in ultrafiltration failure in long run peritoneal dialysis.

Key words

Aquaporin-1 / Endothelial nitric oxide synthase

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LIN Xing-hui;QIAN Jia-qi;NI Zhao-hui;Alessandro Amore;Rosanna Coppo. ffect of glucose degradation products on AQP1 and eNOS expression in cultured endothelial cells[J]. Chinese Journal of Blood Purification. 2008, 7(8): 424-426