Abstract: 【Abstract】 Objective To screen out a semi-permeable membrane with the best hepatic-compatibility suitable for making hollow fiber bioreactor in bioartificial liver supporting system. Methods Human liver cell lines (L02) were cultured on 6 different semi-permeable membranes made of polyethersulfone (PES), polysulfone (PSF), cellulose acetate (CA), polyvinylidene fluoride (PVDF), polypropylene (PP) and cellulose nitrate acetate (CNA). The cells cultured on polystyrene plate were used as a control group. Cytotoxicity of the membrane was evaluated by the data of lactate dehydrogenase (LDH) leakage and DNA ladder. Adherence ratio, cell viability, protein synthesis and DNA amount of the L02 cells were determined. Results No cytotoxicity was found to the L02 cells growing on the 6 membranes. The adherence ratio of L02 cells cultured on PES membrane was significantly higher than that of the cells cultured on CA, PVDF, PP and CAN membranes, and was similar to that of the control group and the cells cultured on PSF membrane. The amount of total DNA from L02 cells growing on PES and PSF membranes was higher than that from the cells growing on CA, PP and CNA membranes. On the 7th day of culture, the cell viability was higher in cells growing on PES membrane than those on CA, PVDF, PP and CAN membranes. Conclusion PES membrane showed the best hepatic-compatibility among the 6 membranes tested. Therefore, PES is suitable for making hollow fiber bioreactor in bioartificial liver supporting system.

Key words: Hepatocytes, Compatibility, Hollow fiber