Construction of bioartificial renal tubule assist device and effect of this device on serum interleukin-6, tumor necrosis factor-αnd interleukin-10 in pigs with multiple organ dysfunction syndrome and acute renal failure

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Chinese Journal of Blood Purification ›› 2009, Vol. 8 ›› Issue (1) : 46-48.

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Abstract

【Abstract】 Object To construct bioartificial renal tubule assist device (RAD) and to investigate the effect of this device on inflammation processes in pigs suffering from multiple organ dysfunction syndrome and acute renal failure (ARF-MODS). Method The bioartificial renal tubule assist device was fabricated using porcine renal proximal tubule cells growing in an AV-400S hemofilter. ARF-MODS pigs (n=20) were induced by incarceration of bilateral renal arteries, ligation and perforation of cecum. The animals were then randomly divided into two groups: continuous venovenous hemofiltration (CVVH) treated group (n=6) and CVVH+RAD treated group (n=6). Their survival time was calculated. Serum IL-6, TNF-α and IL-10 were measured by ELISA at 0, 12 and 24 hours after treatment. Results (a) The inulin recuperative rate of RAD was 0.965±0.027 by spot check. Microscopy revealed that the cells cover the inner surface of the hollow fibers almost completely. (b) The average survival time in CVVH+RAD treated group was 36.82±10.78 hours, longer than that in CVVH treated group (28.46±9.82 hours, P＜0.05). (c) In the CVVH+RAD and CVVH groups, serum IL-10 was higher at 24 hours after treatment than at 0 hour (P＜0.05), especially in the former group at 24 hours after the treatment (P＜0.05). Serum IL-6 and TNF-α decreased in the 2 groups after treatment for 24 hours (P＜0.05), but the changes of the 2 cytokines had no statistical differences between the 2 groups. Conclusion CVVH+RAD treatment was superior to CVVH treatment for ARF-MODS pigs. RAD treatment increased the serum anti-inflammatory factor IL-10, and maintained the balance between inflammatory factors and anti-inflammatory factors, which may be the mechanisms to be effective in ARF-MODS pigs.