【Abstarct】 Objective To observe the effect of lower temperature dialysate for hemodialysis in children. Methods We analyzed 95 times of hemodialysis using 37℃ dialysate or 35℃ dialysate for children with acute or chronic renal failure. Their blood pressure, heart rate and the incidence of hypotension were compared. Results After hemodialysis for 3 hours, the children treated with 35℃ dialysate showed higher diastolic blood pressure than those treated with 37℃ dialysate (P=0.023). The decrease of diastolic and mean blood pressure was greater in children treated with 37℃ dialysate than in those with 35℃ dialysate, and these differences became more apparent after the hemodialysis for 3 hours. In children treated with 35℃ dialysate, heart rate was relatively stable (P=0.032), but without the advantages in hypotension incidence and dialysis efficiency. Conclusion Lower temperature hemodialysate is better in maintaining hemodynamic stability, and is also suitable for children.