【Abstract】 Objective To investigate if the original electrodes can be replaced by China-made electrodes for the measurement of extracellular water (ECW) and intracellular water (ICW) by bioimpedance. Methods Twelve healthy subjects and 23 patients on maintenance hemodialysis (MHD) were included in this study. Examinees were asked to keep in a supine position for at least 5 minutes. Xitron surface bioimpedance analyzer was used to measure resistance and reactance under different frequencies. ECW and ICW were calculated using the software provided by Xitron. The examination for an examinee included ECW and ICW measurements using the electrodes in the original instrument package and the electrodes made in china. The examination was performed once for healthy subjects, and performed once before dialysis session and once after the session for MHD patients. Results were compared using paired t test and Bland-Altman plot. A P value less than 0.05 was considered to be statistically significant. The absolute value of a mean difference and its standard deviation less than 0.5L was considered to be acceptable. Results ECW measured by original electrodes was (15.41±4.53)L, while by China-made electrodes was (15.18±4.46)L; ICW measured by original electrodes was (17.87±6.03)L, while by China-made electrodes was (19.93±6.09)L. The mean difference was (-0.23±0.43)L for ECW, and was (2.06±3.05)L for ICW. Conclusion The China-made electrodes can replace the original electrodes for ECW but not for ICW measurement.