【Abstract】 Objective To explore the influence of glucose-added dialysate on incidences of intradialytic hypoglycemia and hypotension and on metabolic parameters in hemodialysis patients with diabetic nephropathy. Methods Self-control study was conducted in 17 patients with diabetic nephropathy on hemodialysis. The patients were dialyzed with glucose-free dialysate for a period of 2 months, and then were changed to glucose-added dialysate for another period of 2 months. The incidences of intradialytic hypoglycemia and hypotension and the changes of metabolic parameters were compared between the two periods. Results The incidences of intradialytic hypoglycemia and hypotension were 7.6% and 18.3%, respectively, after the period using glucose-free dialysate; the incidences decreased to 1% (P<0.01) and 10.7% (P<0.05), respectively, after the period using glucose-added dialysate. Metabolic parameters including glycosylated hemoglobin and blood lipid profiles remained unchanged (P>0.05). Conclusion Hemodialysis with glucose-added dialysate is beneficial to maintain normal blood glucose and blood pressure in patients with diabetic nephropathy during hemodialysis, but their metabolic parameters remain unchanged.