【Abstract】Objective Heart rate variability (HRV) is related to autonomic nervous system disturbance. The aim of current study was to evaluate whether dialysates with glucose (DG) and dialysate without glucose (DNG) affect HRV, blood pressure and blood glucose during hemodialysis (HD) sessions. Methods Ten stable nondiabetic chronic HD patients were enrolled in this study. Each patient underwent 4 HD sessions, of which twice used DG and twice used DNG. Patients were dialyzed for 4 hours per session. During every dialysis session, heart rate was recorded continuously; blood pressure and blood glucose were measured at 0, 1st, 2nd, 3rd and 4th hour in the session. The influence of DG on HRV index, blood pressure and blood glucose was studied. Analysis of variance for repeated measurements was used for statistical analysis. Results Baseline heart rate and blood pressure were comparable when patients used either DNG or DG. During HD sessions, systolic arterial pressure was significantly higher when patients used DNG than they used DG (F=1718.10, P＜0.05). The occurrence of hypoglycemia was less when patients used DG. In patients using DNG, normalized low-frequency was significantly higher while normalized high-frequency was lower, compared with those when using DG (P＜0.05). The low-frequency/high-frequency ratio was higher in patients when using DNG than when using DG, but without statistically significance (t=0.66, P＞0.05). Conclusion Hemodialysis using DNG may cause hyperactivity of sympathetic nerve system and hence hypertension during HD session. HD patients may benefit from glucose containing dialysate.