【Abstract】 Objective To investigate the effect of nursing intervention (improved preoperative instruction mode) before renal puncture biopsy in reducing postoperative dysuresia on bed. Method We prospectively observed the prevalence of postoperative dysuresia, and compared the prevalence rate before and after nursing intervention. A total of 481 patients with renal puncture biopsy were enrolled in this study. The intervention group included 245 patients admitted for renal puncture biopsy in the period of Jan. to Dec. 2007, and were given instructions following improved preoperative instruction mode. The 236 patients in the control group were admitted for renal puncture biopsy during Jan. to Dec. 2007, and were given routine preoperative oral instructions. Results There were no significant differences in age and gender between the two groups (P>0.05). The prevalence of postoperative dysuresia on bed was 5.51% (13/236) in the control group and 2.45% (6/245) in the intervention group (&#61539;2=4.267, P<0.05). Conclusions Nursing intervention with standard, precise, and descriptive instructions of improved instruction mode can reduce the prevalence of dysuresia on bed after renal puncture biopsy.