Abstract: 【Abstract】Objective To evaluate the efficacy and safety of the disinfectant Sanacide for overnight dwelling disinfection of dialysis machines. Methods Five Nikkiso DBB-27 dialysis machines were used for the test. The disinfectant was diluted by 100 times and then used for overnight dwelling disinfection of the machines after daily running. Concentration of the disinfectant was assayed in the disinfection period. Results of Sudan black B, Ponceau 3R and periodic acid-schiff (PAS) staining for water in circuit were compared before and after the disinfection. After disinfection, residual concentration of the disinfectant was measured to evaluate its safety. Contents of endotoxin and bacteria were measured in water at the inlet of the machine during wash the circuit every month and in dialysate before and after the endotoxin filter to evaluate the effectiveness of the disinfectant. Results After 15 min, the effective concentrations of peracetic acid and hydrogen peroxide stabilized at the highest concentration of 190 ppm and 600 ppm, respectively. The residual concentration after disinfection was <0.02 ppm. The endotoxin level and bacterial colonies in water at the inlet of dialysis machine were <0.01 EU/mL and <10 CFU/ml, respectively. The endotoxin level in water before dialysate filter was below the minimum detection accuracy of the instrument, and bacterial colonies were <0.1 CFU/ml. The endotoxin level in water after dialysate filter was below the minimum detection accuracy of the instrument, and the bacterial colonies were 0 CFU/ml. Conclusion It is safe and effective to use Sanacide for overnight dwelling disinfection of dialysis machines.

Key words: Dialysis Machine, Disinfection, Overnight Disinfection