Abstract: Objective To investigate the significance of CD4+CD127- regulatory T cells in peripheral blood of maintenance hemodialysis patients. Methods The peripheral blood samples were collected from 13 maintenance hemodialysis patients, before trans-analysis and after. The CD4+CD127-Treg and CD4+CD25+CD127-Treg ratios in CD4+T were detected by using flow cytometry. Results The CD4+T ratios in lymphocyte of maintenance hemodialysis patients were higher than healthy controls group before trans-analysis and the CD4+CD127-Treg and CD4+CD25+CD127-Treg ratios in CD4+T were lower, but CD4+T ratios decreased and CD4+CD25+CD127-Treg ratios increased after trans-analysis. Conclusion The number of CD4+T is increasing, CD4+CD127-Treg is decreasing in maintenance hemodialysis patients and their immune is disturbing, and hemodialysis can improve immune function of the patients.

Key words: CD4+CD127-regulatory T cells, Flow cytometer