Abstract: Object To establish the rapid determination of blood atropine and to study the clearance rate of blood atropine by hemoperfusion. Method We performed perfusion and adsorption for healthy sheep blood mixed with atropine through an extracorporeal closed circulating perfusion apparatus loaded with membrane-coated activated charcoal, similar to that for clinical use. Blood atropine was determined by high performance liquid chromatography (HPLC). Result The recovery rate of low, middle and high concentrations of blood atropine ranged 107.0 ~ 110.7%, with the relative standard deviation (RSD) of 0.95 ~ 7.8%. Blood atropine was 74.51μg/ml before hemoperfusion. After perfusion for 1.0h, 2.0h and 3.0h, blood atropine was 16.00μg/ml, 3.85μg/ml and 1.51μg/ml, respectively, in the 0.5g group, decreased to 6.45μg/ml, 0.81μg/ml, 0.21μg/ml, respectively, in the 1.0g group, and was 4.50μg/ml, 0.43μg/ml, 0.32μg/ml, respectively, in the 1.5g group. Conclusion Blood atropine can be quickly and accurately determined by HPLC. Hemoperfusion with membrane-coated activated charcoal can also remove atropine sulfate efficiently. Therefore, blood atropine must be monitored, and appropriate dose of atropine must be supplemented in time in clinical hemoperfusion.

Key words: Membrane coated activated charcoal, Atropine sulphate, HPLC