Abstract: 【Abstract】 Objective To study the effects of advanced glycation end products-human serum albumin (AGE-HSA) on the production of monocyte chemoattractant protein-1 (MCP-1) in human peritoneal mesothelial cells (HPMC) and the role of reactive oxygen species (ROS) in this process. Methods AGE-HSA (0, 100, 500 and 1000μg/mL) with or without 30mM N-acetyl-L-cysteine (NAC) were added to the cell culture medium to stimulate HPMC. MCP-1 mRNA was measured by semi-quantitative RT-PCR, and MCP-1 protein in HPMC was determined by ELISA. The cells were marked with oxidation–susceptible flurorescent probe 2,7-dichorofluoresin diacetate (DCFH) and then were assayed by flow cytometry. Results AGE-HSA increased the concentration of ROS in HPMC with a dose-dependent manner. AGE-HSA also stimulated the production of MCP-1 in dose- and time- dependent manners. The effects of AGE-HSA on HPMC could be blocked by antioxidant NAC. Conclusion The induction of ROS by AGE-HSA in HPMC stimulates the expression of MCP-1. This process may play an important role in the inflammatory reaction and may lead to the ultrafiltration failure.

Key words: Reactive oxygen species, MCP-1, Peritoneal dialysis