【Abstract】Objective To investigate the effect of oxygen therapy combined with Hemodialysis (HD) on dialysis hypotension (IDH). Methods Eighty-four hemodialysis patients were selected and divided into two stages by self-control method. Stage I was treated with simple hypothermic hemodialysis, stage II oxygen therapy combined with hypothermic hemodialysis for 8 weeks. Two phases monitor the frequency of hypotension during dialysis and detect two-stage biochemical and blood gas analysis parameters. Results Oxygen therapy combined with hypothermic hemodialysis significantly reduced the number of episodes of IDH (from 3.16±2.57 to 1.98±1.84, t=4.030, P=0.001). There was a small difference in serum albumin between the two stages but it was statistically significant [stages I and II were (33.2±2.9) and (32.3±2.5)g/L, t=2.154, P=0.033]. The blood gas analysis showed that compared with the no oxygen therapy stage, Oxygen therapy significantly reduced TCO2 (t =8.765, P＜0.001) and HCO3- (t=10.820, P＜0.001) and increased pH (t=18.330, P＜0.001). Conclusion Oxygen therapy combined with hypothermic hemodialysis has a better effect on IDH and can maintain the metabolic acid-base balance in hemodialysis to a certain extent.