Chinese Journal of Blood Purification

12 December 2008, Volume 7 Issue 12 Previous Issue    Next Issue

专家述评

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Please take into consideration concerning blood purification in liver disease treatment DUAN Zhong-ping 2008, 7 (12):  639-640.  Abstract ( 473 )   HTML ( 0 )   PDF (227KB) ( 260 )   Metrics

基础研究

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Experimental study on CA/PEI membrane to the factors relating to liver diseases along with the clearance of bilirubin LI Lei-lei;DUAN Zhi-jun;JU Jia 2008, 7 (12):  641-643.  Abstract ( 643 )   HTML ( 0 )   PDF (379KB) ( 225 )   Objective To study the effect of CA/PEI membrane on cytokines, immunoglubin and complements along with bilirubin clearance in chronic liver diseases. Methods We used CA/PEI membrane to adsorb jaundice sera from 30 cases for 2 hours. Serum IL-6, IL-10, TNF-α and TGF-β1 were measured by ELISA before and after the adsorption. IgA, IgM, IgG, C3 and C4 were detected by immune scatter turbidimetry. Results After the adsorption, serum IL-6, TNF-α and TGF-β1 decreased (P＜0.05), and IL-10, immunoglobulin and complements remained unchanged. Conclusion CA/PEI membrane may affect several cytokines relating to the progress of liver diseases in company with bilirubin clearance. Metrics

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The adsorption of bilirubin by PTFE fiber membrane attached with BSA on its outer layer: Apreliminary experimental study WANG Yong;LU Wei;ZHANG Zhen-pu 2008, 7 (12):  644-646.  Abstract ( 694 )   HTML ( 0 )   PDF (357KB) ( 251 )   Objectives To study the effect of PTFE hollow fiber tube attached with bovine serum albumin (BSA) on its outer layer on the adsorption of bilirubin. Methods For bilirubin adsorption test, we selected glytidy methacrylate (GMA) of 35% grafted rate and attached with BSA 75 mg per gram fiber MPTFE, centrifugal casting of the legal system into a plasma filter, having about 0.35m2 of filter area, and under the conditions of dark, 37℃ and 150-250ml/min plasma flow rate. Plasma circulated in the fiber tube. Due to the concentration gradient of bilirubin exterior and interior of PTFE tube, bound bilirubin in plasma dissociates from albumin, passes through the fenestrated membrane, and rebinds the BSA on the outer wall of the membrane, resulting in a new balanced bilirubin concentration across the membrane. We also observed the changes of plasma bilirubin concentration along with time. Results After the dialysis, plasma bilirubin concentration decreased gradually and significantly, reached the balanced concentration after 100 minutes with the bilirubin adsorption rate of about 15.26-32.11%. Conclusion The PTFE hollow fiber tube attached with bovine serum albumin (BSA) on its outer layer filters and adsorbs plasma bilirubin in the tube, resulting in the decrease of plasma bilirubin. The treated plasma is isolated from other plasma, and this method is therefore simpler than that using molecular absorption of recycling system (MARS). Dialysis using this tube is safer than the detoxication and adsorption procedure using plasma perfusion. Metrics

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The effect of hepatocyte growth factor in the prevention and treatment of peritoneal fibrosis in peritoneal dialysis DU Ye-hui;BIAN Xiao-hui 2008, 7 (12):  647-650.  Abstract ( 694 )   HTML ( 0 )   PDF (406KB) ( 259 )   Objective To explore the effect and mechanism of hepatocyte growth factor in the prevention and treatment of peritoneal fibrosis. Method MTT method was used to measure the proliferation of cultured rat peritoneal mesothelial cells (RPMC) after stimulation by hepatocyte growth factor. ELISA was applied to examine the quantity of FN, TGF-β1 and PAI-1 in the cultured supernatants. RT-PCR was used to detect the expression of FN mRNA, PAI-1 mRNA, and TGF-β1 mRNA in RPMC. Results Hepatocyte growth factor (>30ng/ml) improved the inhibition of RPMC proliferation induced by high glucose. In RPMC incubated in dialysate containing high glucose (4.25%), FN, TGF-β1 and PAI-1 in cultured medium increased significantly, and FN, TGF-β1 and PAI-1 mRNA in these cells also increased. In RPMC incubated with hepatocyte growth factor, however, FN, TGF-β1 and PAI-1 in cultured medium decreased with the down-regulation of FN, TGF-β1 and PAI-1 mRNA in these cells. Conclusion Hepatocyte growth factor improves the inhibition of RPMC proliferation induced by high glucose, and down-regulates the expression of FN, TGF-β1 and PAI-1 in RPMC. Metrics

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A preliminary study of polyethersulfone membrane with a single-layer skin supporting the growth of HepG2 cells YANG Guang-hui;ZHANG Shi-chang;WANG Ying-jie;LIU Tao;SUN Shu-dong 2008, 7 (12):  651-654.  Abstract ( 681 )   HTML ( 2 )   PDF (514KB) ( 191 )   Objective To evaluate whether polyethersulfone (PES) membrane is capable of supporting hepatocyte growth and suitable to be used as the bioartificial liver support systems. Methods The structure of PES plate membrane was observed microscopically. HepG2 cells grew on the PES membrane, and their morphology, growth curve, albumin secretion and the transformation by pentoxyresorufin were examined. Results Prepared PES plate membrane demonstrated a single-layer skin, porous and asymmetric structure. HepG2 cells cultured on PES membrane showed a typical growth curve and proliferated to 6-7 folds after 8 days. These cells clustered together and covered the surface of the membrane, even extended into the micropores. LDH leakage had no difference between the HepG2 cells growing on the membrane and those of the control group. HepG2 cells growing on the membrane showed higher protein biosynthesis and biotransformation functions. Conclusion The PES membrane is a substrate suitable for hepatocytes growing on it. It may be useful for the construction of hollow fiber bioreactors. Metrics

人工肝

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Effect and significance of plasma exchange on T lymphocyte subsets in chronic severe hepatitis patients infected with HBV LIU Fu-hui;HOU Qing-shun;ZHANG Hai-feng;LIU Yong-peng;SUN Shu-lun 2008, 7 (12):  655-656.  Abstract ( 724 )   HTML ( 0 )   PDF (308KB) ( 221 )   Objective To evaluate the effect and significance of plasma exchange on T-Lymphocyte subsets in chronic severe hepatitis patients infected with HBV (CSHB). Methods We recruited 26 cases of CSHB treated with plasma exchange and 47 cases of chronic HBV hepatits (CHB) in this study. The total number and percentage of T-lymphocyte subsets in peripheral blood before and after plasma exchange were detected in CSHB and CHB patients by a FACSCalibur flow cytometer. The differences between CSHB and CHB patients and between recover group and death group in CSHB patients were analyzed. Results Total number of T-lymphocytes and percentage of CD4+ cells were significantly lower, and percentage of CD8+ cells was significantly higher in CSHB patients than in CHB patients (P＜0.01). In CSHB patients, the survival rate in the 3 months after plasma exchange was 53.85% (14/26). In survival CSHB patients after plasma exchange, the total number of T-lymphocytes and percentage of CD4+ cells were significantly higher, and percentage of CD8+ cells was significantly lower than those before the treatment and those in death patients after plasma exchange (P＜0.05 or 0.005). Conclusions The derangement of immune function in CSHB patients can be improved by plasma exchange. The examination of immune function is helpful for the evaluation of prognosis and therapeutic effects in CSHB patients. Metrics

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Comparison study on the adsorption of bilirubin, bile acids and blood ammonia in plasma samples from live failure patients by different adsorbents CHEN Xing;LIU Xin-yu;PENG You-ming;ZHANG Lei;LIU Fu-you 2008, 7 (12):  657-660.  Abstract ( 765 )   HTML ( 1 )   PDF (351KB) ( 284 )   Objective To compare the adsorption of bilirubin, bile acids and blood ammonia in plasma samples from hepatic failure patients by different adsorbents. Methods We collected plasma samples from 4 hepatic failure patients, and divided each sample into 6 parts for 6 adsorbents: HA330 macroporous resin (HA330), activated charcoal encapsulated with HSA (activated charcoal + HSA), AB-8 macroporous resin (AB-8), AB-8 macroporous with hydroxy and diamine arms and coating with HAS (treated AB-8+HSA), ethyl cellulose coating with HAS (EC+HSA) and ethyl cellulose with hydroxy and diamine arms encapsulated with HAS (treated EC+HSA). We then observed the adsorption of bilirubin, bile acids and blood ammonia in the plasma samples passed through the adsorbents. Results The adsorbents effectively adsorbed bilirubin and bile acids in the plasma samples (P＜0.05) except the adsorbent EC+HAS. Among the adsorbents we used, treated EC+HAS had the highest efficiency of bilirubin adsorption (10.82 ± 1.24), and activated charcoal + HSA showed the best ability to adsorb bile acids (9.85 ± 1.50). All of the 6 adsorbents adsorbed blood ammonia (P＜0.05) with the highest capability using treated EC+HSA (33.83±2.51). Conclusion Treated EC+HSA is the best adsorbent for the adsorption of bilirubin, bile acids and blood ammonia. Metrics

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Experiences of plasmapheresis in the treatment of severe and critical diseases in children LIU Xiao-mei;SHEN Ying;MENG Qun 2008, 7 (12):  661-663.  Abstract ( 662 )   HTML ( 0 )   PDF (342KB) ( 235 )   Objective To explore clinical therapeutic effect and program of plasmapheresis in the treatment of critical diseases in children. Methods We retrospectively studied the effectiveness of plasmapheresis in 15 patients (22 months to 15 years old, mean 6.8 years) with severe and critical diseases. Plasmapheresis was performed by the PRISMA machine and the TPE2000 membrane plasma separator using freshly frozen plasma as the exchanger. The average total exchange volume was 40-70ml/kg, achieved a blood flow rate of 50-120ml/min over the duration of 2-3 hours. Results We successfully carried out 39 times of plasmapheresis for 15 severe and critical children without any apparent complications. After plasmapheresis, clinical status and biochemistry parameters improved in 14 children, and full recovery gained in 5 cases. Conclusion Plasmapheresis appears to be safe and effective for the treatment of severe and critical diseases in children. Metrics