中国血液净化

中国血液净化 ›› 2018, Vol. 17 ›› Issue (03): 156-159.doi: 10.3969/j.issn.1671-4091.2018.03.003

• 临床研究 • 上一篇    下一篇

维持性血液透析患者检测miR-126 的临床意义

阿地拉·阿布里孜1,热孜万古丽·阿布都拉1,王昌敏2,田刚3   

  1. 新疆维吾尔自治区人民医院1肾病科2检验科 3天津市公安医院检验科
  • 收稿日期:2017-07-28 修回日期:2018-01-10 出版日期:2018-03-12 发布日期:2018-03-12
  • 通讯作者: 田刚tiangang@126.com E-mail:tiangang_chn@126.com

The clinical significance of miR-126 in maintenance hemodialysis patients

  • Received:2017-07-28 Revised:2018-01-10 Online:2018-03-12 Published:2018-03-12

PDF

627

摘要： 【摘要】目的探讨维持性血液透析患者检测微核糖核酸126(micro ribonucleic acid 126, miR-126)的临床意义。方法60 例维持性血液透析患者中45 例并发冠心病，分为稳定性心绞痛15 例(Ⅰ组)、不稳定性心绞痛12 例(Ⅱ组)、非ST 段抬高性心肌梗死10 例(Ⅲ组)和ST 段抬高性心肌梗死8 例(Ⅳ组)。无并发冠心病组15 例。正常对照组40 例。维持性血液透析患者采用颈部动脉彩超检查颈动脉内-中膜厚度(intimal-medial wall thickness,IMT)和有无斑块，分为正常无增厚A 组，内膜增厚患者B 组，斑块形成患者C 组和管腔狭窄患者D 组。所有研究对象应用实时定量聚合酶链反应检测miR-126 表达水平，并进行统计学分析。结果对照组与维持性血液透析患者并发冠心病组miR-126 表达水平比较差异有统计学意义最小显著性差异(least—significant difference，LSD)-t=8.553，P＜0.001)，维持性血液透析患者有无并发冠心病组间比较差异有统计学意义(LSD-t=8.024，P＜0.001)。A 组到D 组miR-126 表达水平差别有统计学意义(F=11.219，P＜0.001)。4 组之间除A 组和B 组(LSD-t=0.203，P=1.252)，C 组和D 组(LSD-t=0.193，P=1.342)比较差别无统计学意义外，其它组间比较差别均有统计学意义。Ⅰ组到Ⅳ组miR- 126 表达水平差异有统计学意义(F=6.342，P＜0.001)。4 组之间除Ⅰ组和Ⅱ组(LSD-t=0.173，P=1.349)，Ⅲ组和Ⅳ组(LSD-t=0.225，P=1.024)比较差异无统计学意义外，其它组间比较差异均有统计学意义。冠心病不同类型组miR-126 表达水平与低密度脂蛋白胆固醇含量呈显著负相关(r=-0.358，P=0.031)；而与高密度脂蛋白胆固醇含量呈显著正相关(r=0.405，P=0.029)。结论miR-126检测可提示维持性血液透患者是否并发冠心病，对不同类型冠心病诊断有帮助。

关键词: 维持性血液透析, 冠心病, miR-126

Abstract: 【Abstract】Objective To investigate the clinical significance of miR-126 in patients on maintenance hemodialysis (MHD). Method In the 60 MHD patients, we found 45 cases complicated with coronary artery disease, including stable angina (n=15, group 1), unstable angina (n=12, group 2), non-ST segment elevated myocardial infarction (n=10, group 3) and ST segment elevated myocardial infarction (n=8, group 4), as well as 15 cases without coronary artery disease. Forty healthy people were recruited as controls. The 60 MHD patients were undergone ultrasonography to examine internal carotid arteies and to measure intimal-medial thickness (IMT) of internal carotid arteries. They were then divided in to group A (normal IMT, n=8), group B (abnormal IMT, n=7), group C (abnormal IMT with plaque, n=28), and group D (narrow vessel lumen, n=17). miR-126 was determined by real-time fluorescence quantitative PCR. Results There were significant differences in miR-126 expression level among MHD patients with and without coronary artery disease and normal controls (LSD, t=8.553, P=0.000), and between MHD patients with and without coronary artery disease (LSD, t=8.024, P=0.000). miR-126 expression level was statistically different among groups A, B, C and D (F=11.219, P=0.000) and between 2 of the 4 groups except for comparisons between groups A and B (LSD, t=0.203, P=1.252) and between groups C and D (LSD, t=0.193, P=1.342). miR-126 expression level was also statistically different among groups 1, 2, 3 and 4 (F=6.342, P=0.000) and between 2 of the 4 groups except for comparison between groups 1 and 2 (LSD, t=0.173, P=1.349) and between groups 3 and 4 (LSD, t=0.225, P=1.024). miR-126 expression level was negatively correlated with LDL-C (r=-0.358, P=0.031) and positively correlated with HDL-C (r=0.405, P=0.029). Conclusion Assay of miR-126 expression level is useful to suggest the complication of coronary artery disease and its clinical type in MHD patients.

Key words: Maintenance Hemodialysis, coronary artery disease, miR-126