›› 2011, Vol. 10 ›› Issue (11): 583-587.doi: 10.3969/j.issn.1671-4091.2011.11.00

• 临床研究 • 上一篇    下一篇

透析用水的细菌培养方法比较

田 茹 田爱辉 左 力   

  1. 北京大学第一医院肾脏内科(暨肾脏病研究所);首都医科大学附属北京世纪坛医院肾脏内科
  • 收稿日期:2011-03-23 修回日期:1900-01-01 出版日期:2011-11-12 发布日期:2011-11-12
  • 通讯作者: 左力

Comparison of different culture methods for bacterial recovery in dialysis water

TIAN Ru, TIAN Ai-hui, ZUO Li.   

  1. Institute of Nephrology, Peking University First Hospital, Beijing 100034, China
  • Received:2011-03-23 Revised:1900-01-01 Online:2011-11-12 Published:2011-11-12

摘要:

目的 比较几种透析用水细菌培养方法的敏感性,寻找其中方便、快速、敏感性高的方法。方法 在北京市某三甲医院血透中心随机采样透析用水共85份,分别用4种方法进行细菌培养:①哥伦比亚血琼脂平板培养基37癈培养48h、②TSA培养基37癈培养48h、③R2A培养基37癈培养48h或④20癈培养7d,同时设立阳性及阴性对照组。 结果 1.R2A培养基20癈培养7d细菌检出率最高;37癈培养48h细菌检出率降低,但均高于TSA培养基和血琼脂平板培养基。2.EBPG标准方法与选用R2A培养基37癈培养48h的方法具有较好的一致性,可以互相替代。 结论 我们建议采用EBPG建议的透析用水细菌培养方法,或者通过适当提高温度、缩短时间来改进EBPG建议的方法从而更方便临床使用。

关键词: 透析用水, 微生物学, 培养方法

Abstract:

Objective The sensitivity of the four culture methods for bacteria detection in dialysis water was compared to find out the most sensitive one. Methods A total of 85 samples of dialysis water from a blood purification center in Beijing were collected and detected for bacteria by the four culture methods: method A: Columbia blood agar plate incubated at 37℃ for 48 hours, method B: TSA incubated at 37℃ for 48 hours, method C: R2A incubated at 37℃ for 48 hours, and method D: R2A incubated at 20℃ for 7 days. Tap water was used as positive control and water for injection was used as negative control. Results Among the four methods, the number of bacterial colonies was highest by method D and followed by method C. The results from method C were closely consistent with those from method D. Conclusions Method D is the best method for bacteria detection in dialysis water, which is identical to the method recommended by European Best Practice Guideline. Method C can also be used with similar efficiency.

Key words: Microbiology, Culture method