中国血液净化

中国血液净化 ›› 2015, Vol. 14 ›› Issue (12): 736-738.doi: 10.3969/j.issn.1671-4091.2015.12.010

• 基础研究 • 上一篇    下一篇

虾青素对高糖诱导足细胞损伤的影响

吕亭亭,刘海丽,冯晓慧,孙文广,马可,桂定坤,葛声   

  1. 上海交通大学附属第六人民医院临床营养科
  • 收稿日期:2015-06-30 修回日期:2015-08-06 出版日期:2015-12-12 发布日期:2015-12-12
  • 通讯作者: 葛声 gesheng607@126.com E-mail:ywj4169@163.com

Effect of astaxanthin on high glucose induced podocyte injury

  • Received:2015-06-30 Revised:2015-08-06 Online:2015-12-12 Published:2015-12-12

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摘要： 目的利用条件永生型人源足细胞系，探讨虾青素对高糖诱导的足细胞损伤的影响。方法体外培养人肾脏足细胞，按培养条件的不同分为正常葡萄糖浓度组(NG)、高糖组(HG)、高渗组(MA)、高糖+虾青素不同剂量干预组(10-6M，10-5M，10-4M)。干预24h 后收集足细胞。用Annexin V/PI 双染色法，应用流式细胞仪检测虾青素对足细胞凋亡的影响。并采用物理离心的方法检测虾青素对足细胞粘附力的作用。结果HG 组足细胞早期凋亡率较NG 组增高[(7.7±0.9)% vs.(2.7± 0.74)%, P=0.000]，不同剂量虾青素干预组较HG 组足细胞早期凋亡率均下降[(5.7±1.0)%，P=0.017；(5.2±0.7)%，P=0.006；(4.3±0.9)%，P=0.001]，并呈剂量依赖性。高剂量的虾青素可以缓解高糖诱导的足细胞粘附力的降低[(67.2±4.3)% vs.(37.9±9)%,P =0.017]。结论虾青素对高糖诱导的人足细胞早期凋亡和粘附功能有保护作用。

关键词: 人足细胞, 虾青素, 凋亡

Abstract: Objective To investigate the effects of astaxanthin on high glucose induced lesion in human conditional immortalized podocytes. Methods Human podocytes were cultured in vitro. According to the culture conditions, human podocytes were divided into normal glucose group (NG), high glucose group (HG),
high permeability group (MA), and HG+astaxanthin (10～6 M, 10～5 M and 10～4 M) groups. Apoptosis of human podocytes in each group was examined by flow cytometry after the culture for 24 hours. Cell adhesion was assayed by centrifugation to determine the effect of astaxanthin on podocyte adhesion. Results The early apoptosis rate of podocytes was higher in HG group than in NG group (7.7±0.9% vs. 2.7±0.74%, P=0.000). Astaxanthin reduced the early apoptosis of podocytes in a dose-dependent manner (5.7±1.0%, P=0.017; 5.2± 0.7%, P=0.006; 4.3±0.9%, P=0.001). High dose of astaxanthin could increase podocyte adhesion (67.2±4.3% vs. 37.9±9%, P=0.017). Conclusion Astaxanthin prevents podocytic apoptosis and reduced adhesion induced by HG.

Key words: Human podocyte, Astaxanthin, Apoptosis