中国血液净化

中国血液净化 ›› 2018, Vol. 17 ›› Issue (09): 613-616.doi: 10.3969/j.issn.1671-4091.2018.09.008

• 基础研究 • 上一篇    下一篇

新型胰蛋白胨葡萄糖培养基用于透析用水细菌检测的临床适用性多中心研究

甘良英1,张晓军2,邓祥3,姜埃利4,左力1   

  1. 1. 北京大学人民医院肾内科
    2. 内蒙古国际蒙医院血液透析中心
    3. 天津市第三中心医院
    4. 天津医科大学第二医院
  • 收稿日期:2018-05-25 修回日期:2018-06-08 出版日期:2018-09-12 发布日期:2018-08-24
  • 通讯作者: 左力zuolimd@hotmail.com E-mail:zuoli@bjmu.edu.cn

A multicenter study on the clinical applicability of a novel tryptone glucose extract agar medium for the detection of bacteria in dialysis water

  • Received:2018-05-25 Revised:2018-06-08 Online:2018-09-12 Published:2018-08-24

PDF

663

摘要： 【摘要】目的明确一种新型胰蛋白胨葡萄糖(tryptone glucose extract agar，TGEA)培养基用于透析用水细菌检测的临床适用性。方法分别在4 家血液透析中心的水处理系统出水口、回水口、血液透析机(进水软管连接到透析机的位置)及稀释后的透析液等4 个点取样，使用TGEA 涂布法(20℃，168h)、TGEA 薄膜过滤法(20℃, 168h)和营养琼脂培养基(37℃，48h)作为对照培养基，以新型TGEA 培养基(20℃，168h)作为验证培养基，进行细菌培养并在培养结束后进行细菌菌落计数。结果TGEA 涂布法、TGEA薄膜法和验证培养基的Log10(菌落计数)3 者间无显著性差异，均高于营养琼脂培养基(F=3.346,P=0.025)；菌落检出率TGEA 涂布法、薄膜法和验证培养基均高于营养琼脂培养基，但多组间比较未见显著性差异(χ2=4.267，P =0.234)；Bland-Altman 分析显示验证培养基与TGEA 涂布法和薄膜法的一致性好；验证培养基与TGEA 涂布法和薄膜法菌落计数比值在0.5～1.5 范围内。结论新型胰蛋白胨葡萄糖培养基与经典TGEA 涂布法和TGEA 薄膜过滤法一致性好，操作简便，全过程可在常规环境下完成，具有良好的临床适用性。

关键词: 新型胰蛋白胨葡萄糖培养基, 临床适用性, 透析用水, 细菌培养

Abstract: 【Abstract】Objective To identify the clinical applicability of a novel tryptone glucose extract agar (TGEA) medium for the detection of bacteria in dialysis water. Methods Water samples were collected from the four hemodialysis centers at the outlet of water treatment system, backwater point of water treatment system, hemodialysis machine (inlet hose connected to the dialysis machine), and diluted dialysate. Samples were cultured with TGEA medium using spreading method (20 ℃, 168 hours) and TGEA membrane filtration method (20 ℃, 168 hours), and with nutrient agar medium (37 ℃, 48 hours) as the control medium. TGEA medium (20 ℃, 168 hours) was used as a validation medium, and bacterial colonies were counted at the end of the culture. Results The value of log10(colony counts) had no significant differences between the TGEA spreading method, the TGEA membrane filtration method, and the validation medium method, but was higher than that of nutrient agar medium method (F=3.346, P=0.025). The detection rate of bacteria was higher from the three TGEA media than from nutrient agar medium, but P value was greater than 0.05 probably due to the limited sample size (χ2=4.267, P=0.234). Bland-Altman analysis showed that the result from validation medium was consistent with the results from TGEA spreading method and TGEA membrane filtration method. The colony number ratios of validation medium/TGEA spreading method and validation medium/ membrane filtration method were all in the range of 0.5～1.5. Conclusion The novel TGEA medium is well applicable for clinical use. The culture process using the novel TGEA medium is easy to operate in a routine environment.

Key words: novel tryptone glucose extract agar medium, clinical applicabilit, , dialysis water, bacterial culture