目的 为提高透析液中醋酸根和枸橼酸的检测效率，降低检测成本，简化操作流程，建立同一色谱条件下高效液相色谱法(high performance liquid chromatography，HPLC)测定血液透析液及血液透析用浓缩物中醋酸根和枸橼酸含量的检测方法。 方法 采用十八烷基键合的单分散多孔硅胶微球的色谱柱：以50 mmol/L磷酸盐缓冲溶液(pH 2.0)为流动相；流速1.0 ml/min；检测波长210 nm，柱温30℃；对血液透析液及血液透析用浓缩物中醋酸根和枸橼酸进行含量测定分析。 结果 测试结果显示醋酸根线性范围为1～20 mmol/L，r=0.999957，加标回收率为99.07%～103.41%，相对标准偏差(relative standard deviation，RSD)为2.37%(n=3)；枸橼酸线性范围为0.1～15.0 mmol/L，r=0.999966，加标回收率为101.71%～104.49%，RSD为1.42%(n=3)。 结论 本研究方法可在同一色谱条件下简便、准确地测定出血液透析液及透析用浓缩物中醋酸根和枸橼酸的含量，可应用于血液透析液及透析用浓缩物的生产、使用环节的质量控制，提高检测效率。

Objective  To develop a single high-performance liquid chromatography (HPLC) method for simultaneous determination of acetate and citrate in hemodialysis solution and concentrate, thereby improve detection efficiency, reduce cost, and simplify operation the procedure.  Methods  A C18 column (octadecyl-bonded monodisperse porous silica microspheres) was used with 50 mmol/L phosphate buffer (pH 2.0) as the mobile phase. The flow rate was 1.0 mL/min, the detection wavelength was 210 nm, and the column temperature was 30℃. The contents of acetate and citrate in samples were determined. Results Acetate was linear in the range of 1～20 mmol/L(r=0.999957), with recoveries of 99.07%～103.41% and RSD of 2.37% (n=3). Citrate showed good linearity in the range of 0.1～15 mmol/L(r=0.999966), with recoveries of 101.71%～104.49% and RSD of 1.42%(n=3). Conclusion  This single HPLC method is simple, accurate, and suitable for the simultaneous determination of acetate and citrate in hemodialysis solution and concentrate. It can be applied to quality control during production and clinical use, thereby effectively improving detection efficiency.