肝素在高糖影响大鼠腹膜间皮细胞的增殖及其表达白细胞介素-1中的作用

刘作海; 王钢; 车文体; 伍靖武; 眭维国; 晏强; 张清勇

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中国血液净化 ›› 2003, Vol. 2 ›› Issue (5) : 260-263.

基础研究

肝素在高糖影响大鼠腹膜间皮细胞的增殖及其表达白细胞介素-1中的作用

刘作海王钢车文体伍靖武眭维国晏强张清勇

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摘要

目的初步观察肝素在高糖影响大鼠腹膜间皮细胞(Rat peritoneal mesothelial cells, RPMC) 细胞增殖及其表达白细胞介素-1(Interleukin-1, IL-1) 中的作用，从而为持续性非卧床腹膜透析(Continuous ambulatory peritoneal dialysis, CAPD)中肝素的临床应用打下一定的理论基础。方法建立RPMC的体外培养；并用四四氮唑蓝(MTT)细胞增殖实验和酶联免疫吸附方法(ELISA)，来分析肝素对高糖(3.86%葡萄糖)影响RPMC增殖及其分泌IL-1的作用。结果 3.86%葡萄糖能显著抑制RPMC的增殖，而1.25U/ml肝素能部分逆转高糖对RPMC增殖的抑制作用( P<0.01)；3.86%葡萄糖能显著增加 RPMC表达 IL-1β，而1.25U/ml肝素能部分减少高糖增加RPMC表达 IL-1β的作用 (P<0.01)。结论本文结果提示，肝素可能通过逆转高糖对 RPMC的增殖抑制作用及减少高糖增加RPMC表达促炎细胞因子IL-1的作用，而延缓CAPD相关性腹膜纤维化的发生，最终有益于CAPD进行。

Abstract

Objective To investigate the effect of heparin on inhibition of cell proliferation as well as on expression of interleukin-1(IL-1) in RPMC (rat peritoneal mesothelial cells) by high glucose, so as to provide some theoretic basis for the clinical use of heparin during continuous ambulatory peritoneal dialysis (CAPD). Methods RPMC were cultured in vitro and MTT method was processed to observe the effects of both high glucose (3.86%) and heparin (1.25U/ml) on cell proliferation. In addition, ELISA analysis were performed to investigate the change of IL-1 in the culture muli. ResuIts High glucose of 3.86% could significantly inhibit cell proliferation as well as induce the expression of IL-1β in RPMC (P<0.01), which could be partially reversed by heparin of 1.25U/ml (P<0.01). Conclusion Heparin could reverse both inhibition of cell proliferation and induction of pro-inflammatory cytokine IL-1 mediated by high glucose in peritoneal mesothelial cells, and delay the occurrence of CAPD associated peritoneal fibrinosis and being beneficial to the process of CAPD.