细胞间粘附分子-1在腹膜炎症过程中的作用及己酮可可碱的保护效应 

郭志勇; 李保春; 袁伟杰; 崔若兰

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中国血液净化 ›› 2003, Vol. 2 ›› Issue (6) : 319-321.

基础研究

细胞间粘附分子-1在腹膜炎症过程中的作用及己酮可可碱的保护效应 

郭志勇李保春袁伟杰崔若兰

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摘要

目的探讨细胞间粘附分子-1（ICAM-1）在连续不卧床性腹膜透析（CAPD）患者的腹膜炎症过程中的作用及己酮可可碱的保护效应。方法采用体外腹膜间皮细胞（PMC）与巨噬细胞共同培养的方法，观察抗ICAM-1 MAb对巨噬细胞促进PMC增殖的影响及己酮可可碱的保护效应。结果抗ICAM-1 MAb对巨噬细胞促进PMC的增殖具有抑制作用，呈剂量依赖性；己酮可可碱可抑制IFN-α诱导的PMC与巨噬细胞粘附。结论 ICAM-1 参与、影响腹膜炎症的过程，己酮可可碱在腹腔局部防御过程中发挥重要作用。

Abstract

Objective To investigate the role of ICAM-1 in the process of peritoneum inflammation in CAPD patients and protective effects of pentoxifylline PTX.
Methods Peritoneal mesothelial cell(PMC) and macrophage were co-cultured in vitro. We observed the influence of ICAM-1 on activating PMC proliferation by macrophage and protective effect of pentoxifylline. Results The effect of activating PMC proliferation by macrophage was significantly inhibited and dosage-dependent. PTX was found to antagonize IFN-α induced adhesion reaction of PMC and macrophage. Conclusion ICAM-1 plays an important role in pathologic process of peritoneum inflammation. PTX may play an important role in peritoneal local defense and may be a protective agent which have a potential clinical value.