中国血液净化

›› 2003, Vol. 2 ›› Issue (9): 493-496.

• 基础研究 • 上一篇    下一篇

非洛地平对5/6肾大部分切除大鼠心肌细胞L型钙电流的影响

吴宇澄 赵卫红 余多慰   

  1. 210029 南京，南京医科大学第一附属医院肾内科（吴宇澄，赵卫红）；210097 南京， 南京师范大学生命科学学院（余多慰）
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2003-09-19 发布日期:2003-09-19

  • Received:1900-01-01 Revised:1900-01-01 Online:2003-09-19 Published:2003-09-19

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摘要：

目的 探讨非洛地平对5/6肾大部分切除大鼠心肌细胞L型钙电流的影响。方法 5/6肾大 部分切除手术制作肾功能衰竭大鼠模型，使用膜片钳全细胞记录技术记录心肌细胞L型钙电流。
结果 5/6肾切除大鼠心肌细胞L型钙电流明显增大，稳态失活曲线左移。非洛地平能够逆转L型钙电流的增大，但未能逆转通道失活性质的改变。
结论 慢性肾衰竭可能引起心肌细胞钙操纵的改变，非洛地平可以逆转这种改变，还能促进L型钙离子通道的电压依赖性失活。

关键词: L型钙通道, 心肌细胞, 5/6肾大部分切除, 非洛地平, 膜片钳

Abstract:

Objective To investigate the effects of felodipine on L-type calcium currents of rat cardiomyocytes after 5/6 subtotal nephrectomised. Method Animals were divided into three groups as follow: gorup A, 5/6 subtotal nephrectomy(SNx);group B, SNx administrated with 10mg/kg/d of felodipine; gorup C,control.Sprague-dawley rats underwent two-stage SNx; control rats underwent bilateral renal decapsulation. Animals were sacrificed after 12 weeks.Whole-cell patch clamp techniques were used to examine L-type calcium currents(ICa, L)in isolated cardiomyocytes. Results The amplitude of L-type calcium current significantly increased in SNx rats and was blocked by felodipine partly. The potential giving 50%of inactivation(V1/2) moved towards more negative in SNx rats than in control,and it had no change after administrated felodipine. Conclusions The calciumhandling of SNx rats cardiomyocytes changes.Felodipine can reverse this change of calcium handling partly and promote the voltage-dependent inactivation of L-type calcium channel.

Key words: Cardiomyocytes, 5/6 subtotal nephrectomy, Felodipine, Patch clamp